Neuropeptide Y blocks light-induced phase advances but not delays of the circadian activity rhythm in hamsters.

In mammals, the suprachiasmatic nuclei (SCN) are the anatomical site of localization of the light-entrainable circadian clock responsible for the generation of daily rhythms in physiology and behavior. In addition to direct retinohypothalamic innervation, the SCN receive a prominent projection of fibers from the intergeniculate leaflet (IGL) of the thalamus, the geniculohypothalamic tract (GHT), some of which contain the neurotransmitter, neuropeptide Y (NPY). Since the GHT has been suggested to play a role in the modulation of photic entrainment of the SCN circadian clock in rodents, we investigated the effects of local administration of NPY into the region of the SCN on light-induced phase shifts of the free-running activity rhythm in hamsters. Injection of 60 nmol of NPY into the SCN region 10 min prior to light exposure at circadian time 19 completely blocked light-induced phase advances. Similar treatment at circadian time 14 had no significant effect on the magnitude of light-induced phase delays. Injection of NPY at either time point without light exposure did not alter circadian phase. The findings support a modulatory role for NPY in the photic entrainment of the SCN circadian clock.