Uchida S, Yahagi H
Japanese Red Cross Central Blood Center.
Nihon Rinsho. 1997 Sep;55(9):2267-71.
Post-transfusion graft-versus-host disease (PT-GVHD) is a potentially fatal adverse reaction to blood transfusion with a mortality rate of 95 percent or higher. We previously developed a definitive diagnostic test for PT-GVHD using polymerase chain reaction (PCR) amplification of highly polymorphic microsatellite repeat sequences. The results show the replacement of patient original DNA types with donor types on development of PT-GVHD. However, samples of patient blood obtained before transfusion are rarely stored until more than 2 weeks after the transfusion when PT-GVHD is usually suspected. On these occasions, we analyzed PCR amplified fragments of DNA extracted from fingernail clippings to determine the patient original DNA types. As a rapid and easily method, this system could be a useful tool for the clinical diagnosis of PT-GVHD.
输血后移植物抗宿主病（PT-GVHD）是一种潜在致命的输血不良反应，死亡率达95%或更高。我们之前利用聚合酶链反应（PCR）扩增高度多态性微卫星重复序列，开发了一种针对PT-GVHD的确证诊断测试。结果显示，PT-GVHD发生时患者原有的DNA类型被供体类型所取代。然而，输血前采集的患者血液样本很少保存到输血后2周以上，而此时通常会怀疑发生了PT-GVHD。在这些情况下，我们分析了从指甲剪屑中提取的DNA的PCR扩增片段，以确定患者原有的DNA类型。作为一种快速且简便的方法，该系统可能成为PT-GVHD临床诊断的有用工具。
