Roberts J A, Kaack M B, Baskin G, Marklund B I, Normark S
Department of Urology, Tulane Regional Primate Research Center, Covington, Louisiana, USA.
J Urol. 1997 Oct;158(4):1610-3.
This is a study of the interaction of the tip protein of P-fimbriae E. coli, its specific urothelial adhesin, and urothelial receptors for the adhesin. This tip protein has several epitopes that adhere to different isoreceptors containing the urothelial alpha-gal-1-4 beta-gal disaccharide. Renal tubular cells of our monkey model contain the globoside isoreceptor, and thus ureteral inoculation of E. coli with the class II tip protein leads to pyelonephritis. The class III tip protein adheres to the Forssman antigen and causes cystitis in humans.
An E. coli strain, DS17 which originally caused pyelonephritis in a child, is P-fimbriated and contains a class II tip adhesin. A mutant was produced to contain a class III tip adhesin. Eight monkeys had a ureteral inoculation of E. coli DS17 and 4 monkeys with E. coli DS17-1. In addition, we studied in vitro adherence by these strains.
We show that in vitro adherence by the tip protein of P-fimbriae to bladder cells of the monkey occurs by several mechanisms, adhering to specific receptors for the class II and III epitopes of the tip protein as well as by means of type 1 fimbriae. In addition, the PapE protein of the fibrillum of the P-fimbriae adheres to fibronectin. As always, electrostatic and hydrophobic interaction remain important contributions to adherence. E. coli DS17 caused pyelonephritis, but DS17-1 caused cystitis. Bacteriuria was prolonged by DS17 infection.
The site of a urinary tract infection from P-fimbriated E. coli can be predicted by the epitope of the tip protein of P-fimbriae.
本研究旨在探讨大肠杆菌P菌毛尖端蛋白、其特异性尿路上皮黏附素以及该黏附素的尿路上皮受体之间的相互作用。这种尖端蛋白有多个表位，可与含有尿路上皮α - 半乳糖 - 1 - 4β - 半乳糖二糖的不同异受体结合。我们猴子模型的肾小管细胞含有球苷异受体，因此用II类尖端蛋白对大肠杆菌进行输尿管接种会导致肾盂肾炎。III类尖端蛋白可与福斯曼抗原结合并导致人类膀胱炎。
大肠杆菌菌株DS17最初在一名儿童中引起肾盂肾炎，该菌株有P菌毛并含有II类尖端黏附素。制备了一个含有III类尖端黏附素的突变体。八只猴子接受了大肠杆菌DS17的输尿管接种，四只猴子接受了大肠杆菌DS17 - 1的接种。此外，我们研究了这些菌株的体外黏附情况。
我们发现，P菌毛尖端蛋白在体外与猴子膀胱细胞的黏附通过多种机制发生，可与尖端蛋白II类和III类表位的特异性受体结合，也可通过1型菌毛进行黏附。此外，P菌毛纤丝的PapE蛋白可与纤连蛋白结合。一如既往，静电和疏水相互作用对黏附仍有重要作用。大肠杆菌DS17导致肾盂肾炎，而DS17 - 1导致膀胱炎。DS17感染使菌尿期延长。
来自有P菌毛大肠杆菌的尿路感染部位可通过P菌毛尖端蛋白的表位来预测。
