Roberts J A, Marklund B I, Ilver D, Haslam D, Kaack M B, Baskin G, Louis M, Möllby R, Winberg J, Normark S
Department of Urology, Tulane Regional Primate Center, Covington, LA 70433.
Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11889-93. doi: 10.1073/pnas.91.25.11889.
Nonobstructive acute pyelonephritis in humans is most often caused by P-fimbriated Escherichia coli. P-fimbriae are heteropolymeric fibers carrying a Gal(alpha 1-4)Gal-specific PapG adhesin at its distal end. The pyelonephritic strain DS17 expresses P-fimbriae from a single gene cluster. A mutant strain, DS17-8, which expresses P-fimbriae tacking the PapG adhesin, was constructed by allelic replacement introducing a 1-bp deletion early in the papG gene. In cynomolgus monkeys, DS17 and DS17-8 were equally able to cause bladder infection, whereas only the wild-type strain DS17 could cause pyelonephritis as monitored by bacteriological, functional, and histopathological criteria. Since DS17, but not DS17-8, adheres to renal tissue, these data underscore the critical role of microbial adherence to host tissues in infectious disease and strongly suggest that the PapG tip adhesin of P-fimbriae is essential in the pathogenesis of human kidney infection.
人类非梗阻性急性肾盂肾炎最常见的病因是产P菌毛的大肠杆菌。P菌毛是一种异聚纤维，在其远端携带一种Gal(α1-4)Gal特异性PapG黏附素。肾盂肾炎菌株DS17从单个基因簇表达P菌毛。通过在papG基因早期引入1个碱基对缺失的等位基因替换构建了一个表达缺乏PapG黏附素的P菌毛的突变菌株DS17-8。在食蟹猴中，DS17和DS17-8引起膀胱感染的能力相同，而通过细菌学、功能和组织病理学标准监测，只有野生型菌株DS17能引起肾盂肾炎。由于DS17而非DS17-8能黏附于肾组织，这些数据强调了微生物黏附于宿主组织在传染病中的关键作用，并强烈提示P菌毛的PapG尖端黏附素在人类肾脏感染的发病机制中至关重要。
