单克隆抗体E8-18识别山羊支原体山羊肺炎亚种特有的一种整合膜表面蛋白。
Monoclonal antibody E8-18 identifies an integral membrane surface protein unique to Mycoplasma capricolum subsp. capripneumoniae.
作者信息
Rurangirwa F R, Shompole P S, Wambugu A N, Kihara S M, McGuire T C
机构信息
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.
出版信息
Clin Diagn Lab Immunol. 1997 Sep;4(5):615-9. doi: 10.1128/cdli.4.5.615-619.1997.
Abstract
Monoclonal antibody (MAb) E8-18 reacted with four isolates of Mycoplasma capricolum subsp. capripneumoniae in Western blots identifying an epitope on a 24 kDa antigen (p24). MAb E8-18 did not react with 11 isolates belonging to four other Mycoplasma species or subspecies closely related to M. capricolum subsp. capripneumoniae. A combination of trypsin treatment of intact organisms and detergent-phase partitioning revealed p24 to be an integral M. capricolum subsp. capripneumoniae surface membrane protein.
摘要
单克隆抗体（MAb）E8 - 18在蛋白质免疫印迹法中与山羊支原体山羊肺炎亚种的4个分离株发生反应，鉴定出一个位于24 kDa抗原（p24）上的表位。MAb E8 - 18不与属于其他4个支原体物种或亚种且与山羊支原体山羊肺炎亚种密切相关的11个分离株发生反应。完整生物体的胰蛋白酶处理和去污剂相分配相结合显示p24是山羊支原体山羊肺炎亚种的一种完整表面膜蛋白。
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