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生长沉淀试验作为鉴别支原体和无胆甾原体种属的诊断方法。

The growth precipitation test as a diagnostic method for differentiation of mycoplasma and acholeplasma species.

作者信息

Ernø H, Salih M M

出版信息

Acta Vet Scand. 1980;21(4):469-81. doi: 10.1186/BF03546835.

DOI:10.1186/BF03546835
PMID:7223574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8317748/
Abstract

The purpose of this paper is to evaluate the growth precipitation (GP) test for routine identification. The test was performed as described by (1978) which is a modification of the method of (1972). On the basis of examination of 82 strains, using indirect immunofluorescence (IMF) and growth inhibition (GI) as well as GP tests it is concluded that the GP test seems to be very useful for species identification in the genus Acholeplasma, as this method displayed fewer cross-reactions between species than the other 2 tests. When applied to the genus Mycoplasma, however, the GP test is not species-specific, due to cross reactions observed within the group of arginine positive and within the group of glucose and scrum digestion positive species. In the genus Mycoplasma the method can only be used as a screening tool, and final identification is in general based on growth inhibition and immunofluorescence.

摘要

本文旨在评估用于常规鉴定的生长沉淀(GP)试验。该试验按照(1978年)所述进行,此方法是对(1972年)方法的改进。通过对82株菌株进行检测,采用间接免疫荧光(IMF)、生长抑制(GI)以及GP试验,得出结论:GP试验对于无胆甾原体属的菌种鉴定似乎非常有用,因为该方法与其他两种试验相比,种间交叉反应较少。然而,当应用于支原体属时,由于在精氨酸阳性组以及葡萄糖和血清消化阳性组内观察到交叉反应,GP试验并非种特异性的。在支原体属中,该方法仅可作为一种筛选工具,最终鉴定通常基于生长抑制和免疫荧光。

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The growth precipitation test as a diagnostic method for differentiation of mycoplasma and acholeplasma species.生长沉淀试验作为鉴别支原体和无胆甾原体种属的诊断方法。
Acta Vet Scand. 1980;21(4):469-81. doi: 10.1186/BF03546835.
2
[Serologic differentiation between Acholeplasma laidlawii (Mycoplasma laidlawii) and Acholeplasma granularum (Mycoplasma granularum) strains using agglutination and precipitation].[利用凝集反应和沉淀反应对莱氏无胆甾原体(类胸膜肺炎支原体)和颗粒无胆甾原体(颗粒支原体)菌株进行血清学鉴别]
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3
Partial nucleotide sequence similarity within species of Mycoplasma and Acholeplasma.支原体属和无胆甾原体属物种内的部分核苷酸序列相似性。
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Electrophoretic analysis of isoenzymes of mycoplasma species.支原体属同工酶的电泳分析。
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The F38-like group, a new group of caprine mycoplasmas?F38样菌群,一种新的山羊支原体菌群?
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本文引用的文献

1
Mycoplasmas isolated from cattle in Australia.从澳大利亚牛群中分离出的支原体。
Aust Vet J. 1970 Aug;46(8):378-81. doi: 10.1111/j.1751-0813.1970.tb15577.x.
2
Bovine mycoplasmas: a serological study by the metabolic inhibition test.牛支原体:通过代谢抑制试验进行的血清学研究。
Acta Vet Scand. 1973;14(4):511-23.
3
Identification and characterization of Acholeplasma oculusi spec. nov. from the eyes of goats with keratoconjunctivitis.从患有角结膜炎的山羊眼睛中鉴定并表征眼无胆甾原体新种。
Cornell Vet. 1973 Jan;63(1):117-29.
4
Mycoplasma: growth precipitation as a serodiagnostic method.支原体:作为血清诊断方法的生长沉淀法
Appl Microbiol. 1972 Mar;23(3):553-8. doi: 10.1128/am.23.3.553-558.1972.
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[Goat mycoplasma related to Mycoplasma dispar species].与殊异支原体相关的山羊支原体
Rev Elev Med Vet Pays Trop. 1973;26(1):13-26.
6
Modifications of the growth inhibition test and its application to human T-mycoplasmas.生长抑制试验的改良及其在人T支原体中的应用。
Appl Microbiol. 1973 Apr;25(4):528-33. doi: 10.1128/am.25.4.528-533.1973.
7
Direct and indirect immunofluorescence of unfixed and fixed Mycoplasma colonies.未固定和固定的支原体菌落的直接和间接免疫荧光
Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(4):615-22. doi: 10.1111/j.1699-0463.1972.tb00186.x.
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Bovine mycoplasmas: serological studies by double immunodiffusion, growth precipitation and growth inhibition.
Acta Vet Scand. 1973;14(4):524-37.
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The pathology of experimental and natural contagious carine pleuropneumonia in Kenya.肯尼亚实验性和自然传染性猪胸膜肺炎的病理学
Zentralbl Veterinarmed B. 1976 Sep;23(8):652-61. doi: 10.1111/j.1439-0450.1976.tb00703.x.
10
Lipid and lipopolysaccharide composition of Acholeplasma oculi.眼支原体的脂质和脂多糖组成
J Bacteriol. 1979 Aug;139(2):356-61. doi: 10.1128/jb.139.2.356-361.1979.