采用酶联免疫吸附测定法检测疑似流行性多关节炎患者血清中抗罗斯河病毒IgM抗体。
Detection by ELISA of IgM antibodies to Ross River virus in serum from patients with suspected epidemic polyarthritis.
作者信息
Oseni R A, Donaldson M D, Dalglish D A, Aaskov J G
出版信息
Bull World Health Organ. 1983;61(4):703-8.
Abstract
An ELISA method for the detection of IgM antibody to Ross River virus in the serum of patients with suspected epidemic polyarthritis has been developed. None of the reagents is infectious or toxic and the use of purified virus minimizes the risk of non-specific reactions to non-viral products. In a positive reaction the ELISA substrate changes colour from yellow to purple, enabling serum antibody titres to be read by eye.
摘要
已开发出一种酶联免疫吸附测定（ELISA）方法，用于检测疑似流行性多关节炎患者血清中罗斯河病毒的IgM抗体。所有试剂均无传染性或毒性，使用纯化病毒可将对非病毒产物产生非特异性反应的风险降至最低。在阳性反应中，ELISA底物颜色从黄色变为紫色，使血清抗体滴度能够肉眼读取。
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