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Crystal structure of calf spleen purine nucleoside phosphorylase in a complex with hypoxanthine at 2.15 A resolution.小牛脾脏嘌呤核苷磷酸化酶与次黄嘌呤复合物在2.15埃分辨率下的晶体结构。
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MC-Fit: using Monte-Carlo methods to get accurate confidence limits on enzyme parameters.MC-Fit：使用蒙特卡罗方法获得酶参数的准确置信限。
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Comparison of the enzymatic properties of the two Escherichia coli lysyl-tRNA synthetase species.两种大肠杆菌赖氨酰 - tRNA合成酶的酶学性质比较。
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Role of the 1-72 base pair in tRNAs for the activity of Escherichia coli peptidyl-tRNA hydrolase.1-72碱基对在大肠杆菌肽基-tRNA水解酶活性的tRNA中的作用。
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大肠杆菌肽基 - tRNA水解酶的1.2埃分辨率晶体结构及活性位点图谱

Crystal structure at 1.2 A resolution and active site mapping of Escherichia coli peptidyl-tRNA hydrolase.

作者信息

Schmitt E, Mechulam Y, Fromant M, Plateau P, Blanquet S

机构信息

Laboratoire de Biochimie, Unit'e de Recherche Associ'ee No. 1970 du Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau, France.

出版信息

EMBO J. 1997 Aug 1;16(15):4760-9. doi: 10.1093/emboj/16.15.4760.

DOI:10.1093/emboj/16.15.4760
PMID:9303320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1170102/
Abstract

Peptidyl-tRNA hydrolase activity from Escherichia coli ensures the recycling of peptidyl-tRNAs produced through abortion of translation. This activity, which is essential for cell viability, is carried out by a monomeric protein of 193 residues. The structure of crystalline peptidyl-tRNA hydrolase could be solved at 1.2 A resolution. It indicates a single alpha/beta globular domain built around a twisted mixed beta-sheet, similar to the central core of an aminopeptidase from Aeromonas proteolytica. This similarity allowed the characterization by site-directed mutagenesis of several residues of the active site of peptidyl-tRNA hydrolase. These residues, strictly conserved among the known peptidyl-tRNA hydrolase sequences, delineate a channel which, in the crystal, is occupied by the C-end of a neighbouring peptidyl-tRNA hydrolase molecule. Hence, several main chain atoms of three residues belonging to one peptidyl-tRNA hydrolase polypeptide establish contacts inside the active site of another peptidyl-tRNA hydrolase molecule. Such an interaction is assumed to represent the formation of a complex between the enzyme and one product of the catalysed reaction.

摘要

来自大肠杆菌的肽基 - tRNA水解酶活性可确保通过翻译终止产生的肽基 - tRNA得以循环利用。这种对细胞活力至关重要的活性由一种含193个残基的单体蛋白执行。结晶态的肽基 - tRNA水解酶结构能够以1.2埃的分辨率解析出来。它显示出一个围绕扭曲的混合β - 折叠构建的单一α/β球状结构域，类似于解蛋白气单胞菌一种氨肽酶的中心核心。这种相似性使得通过定点诱变对肽基 - tRNA水解酶活性位点的几个残基进行了表征。这些在已知的肽基 - tRNA水解酶序列中严格保守的残基勾勒出一个通道，在晶体中，该通道被相邻肽基 - tRNA水解酶分子的C末端占据。因此，属于一个肽基 - tRNA水解酶多肽的三个残基的几个主链原子在另一个肽基 - tRNA水解酶分子的活性位点内形成接触。这种相互作用被认为代表了酶与催化反应的一种产物之间复合物的形成。