大肠杆菌中一种赋予对头孢霉素耐药性的新型C类β-内酰胺酶（FOX-2）。
A novel class C beta-lactamase (FOX-2) in Escherichia coli conferring resistance to cephamycins.
作者信息
Bauernfeind A, Wagner S, Jungwirth R, Schneider I, Meyer D
机构信息
Max von Pettenkofer-Institut, Munich, Federal Republic of Germany.
出版信息
Antimicrob Agents Chemother. 1997 Sep;41(9):2041-6. doi: 10.1128/AAC.41.9.2041.
Abstract
An Escherichia coli strain resistant to a broad spectrum of beta-lactams, including cephamycins, was isolated from a patient suffering from urinary tract infection. A resistance plasmid (pMVP-7) was transferred from the clinical isolate to an Escherichia coli recipient. Both strains produce a cefoxitin-hydrolyzing beta-lactamase focusing at pI 6.7. The phenotype was similar to that of a Klebsiella pneumoniae strain producing cephamycinase FOX-1, so primers were selected from the FOX-1 sequence to amplify the bla gene of the transconjugant. The PCR product obtained was sequenced. The percentage of identity of the deduced amino acid sequence with sequences of other AmpC-type beta-lactamases was 96.9% with FOX-1, 74.9% with CMY-1, and 67.7% with MOX-1. This new plasmid-mediated enzyme is most closely related to FOX-1 (11 amino acid exchanges). We therefore propose the designation FOX-2.
摘要
从一名患有尿路感染的患者体内分离出一株对包括头孢霉素在内的多种β-内酰胺类抗生素耐药的大肠杆菌菌株。一个耐药质粒（pMVP-7）从临床分离株转移到了大肠杆菌受体菌中。这两种菌株都产生一种聚焦于pI 6.7的水解头孢西丁的β-内酰胺酶。该表型与一株产头孢霉素酶FOX-1的肺炎克雷伯菌菌株相似，因此从FOX-1序列中选择引物来扩增转接合子的bla基因。对获得的PCR产物进行测序。推导的氨基酸序列与其他AmpC型β-内酰胺酶序列的同一性百分比与FOX-1为96.9%，与CMY-1为74.9%，与MOX-1为67.7%。这种新的质粒介导酶与FOX-1关系最为密切（有11个氨基酸交换）。因此，我们提议将其命名为FOX-2。
相似文献
1
A novel class C beta-lactamase (FOX-2) in Escherichia coli conferring resistance to cephamycins.大肠杆菌中一种赋予对头孢霉素耐药性的新型C类β-内酰胺酶（FOX-2）。
Antimicrob Agents Chemother. 1997 Sep;41(9):2041-6. doi: 10.1128/AAC.41.9.2041.
2
Molecular characterization of FOX-4, a new AmpC-type plasmid-mediated beta-lactamase from an Escherichia coli strain isolated in Spain.FOX-4的分子特征分析，FOX-4是一种新的AmpC型质粒介导的β-内酰胺酶，来自于在西班牙分离出的一株大肠杆菌。
Antimicrob Agents Chemother. 2000 Sep;44(9):2549-53. doi: 10.1128/AAC.44.9.2549-2553.2000.
3
Comparative characterization of the cephamycinase blaCMY-1 gene and its relationship with other beta-lactamase genes.头孢菌素酶blaCMY-1基因的比较特征及其与其他β-内酰胺酶基因的关系。
Antimicrob Agents Chemother. 1996 Aug;40(8):1926-30. doi: 10.1128/AAC.40.8.1926.
4
Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC beta-lactamase, and the foss of an outer membrane protein.肺炎克雷伯菌对亚胺培南的耐药性与质粒介导的AmpCβ-内酰胺酶ACT-1和外膜蛋白缺失的联合作用有关。
Antimicrob Agents Chemother. 1997 Mar;41(3):563-9. doi: 10.1128/AAC.41.3.563.
5
Characterization of blaCMY-11, an AmpC-type plasmid-mediated beta-lactamase gene in a Korean clinical isolate of Escherichia coli.韩国临床分离的大肠杆菌中AmpC型质粒介导的β-内酰胺酶基因blaCMY-11的特性分析
J Antimicrob Chemother. 2002 Feb;49(2):269-73. doi: 10.1093/jac/49.2.269.
6
Transferable class C beta-lactamases in Escherichia coli strains isolated in Greek hospitals and characterization of two enzyme variants (LAT-3 and LAT-4) closely related to Citrobacter freundii AmpC beta-lactamase.希腊医院分离出的大肠杆菌菌株中的可转移性C类β-内酰胺酶以及与弗氏柠檬酸杆菌AmpCβ-内酰胺酶密切相关的两种酶变体（LAT-3和LAT-4）的特性
J Antimicrob Chemother. 1998 Oct;42(4):419-25. doi: 10.1093/jac/42.4.419.
7
A novel type of AmpC beta-lactamase, ACC-1, produced by a Klebsiella pneumoniae strain causing nosocomial pneumonia.一种由肺炎克雷伯菌菌株产生的新型AmpCβ-内酰胺酶ACC-1，该菌株导致医院获得性肺炎。
Antimicrob Agents Chemother. 1999 Aug;43(8):1924-31. doi: 10.1128/AAC.43.8.1924.
8
Characterization of a novel AmpC-type plasmid-mediated beta-lactamase from an Escherichia coli strain isolated in China.对从中国分离的一株大肠杆菌中一种新型AmpC型质粒介导的β-内酰胺酶的特性分析
Curr Microbiol. 2008 Dec;57(6):558-63. doi: 10.1007/s00284-008-9242-5. Epub 2008 Sep 10.
9
[Plasmid-mediated cephalosporinase among extended-spectrum-beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae].产超广谱β-内酰胺酶的大肠埃希菌和肺炎克雷伯菌中的质粒介导头孢菌素酶
Zhonghua Nei Ke Za Zhi. 2004 Jul;43(7):487-90.
10
Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants.肺炎克雷伯菌中FOX-1的基因序列和生化特性，一种具有两种分子变体的新型AmpC型质粒介导的β-内酰胺酶。
Antimicrob Agents Chemother. 1994 Sep;38(9):2150-7. doi: 10.1128/AAC.38.9.2150.
引用本文的文献
1
Class C β-Lactamases: Molecular Characteristics.C 类β-内酰胺酶：分子特征。
Clin Microbiol Rev. 2022 Sep 21;35(3):e0015021. doi: 10.1128/cmr.00150-21. Epub 2022 Apr 18.
2
Genetics of Acquired Antibiotic Resistance Genes in spp.[具体物种]中获得性抗生素抗性基因的遗传学
Front Microbiol. 2020 Feb 21;11:256. doi: 10.3389/fmicb.2020.00256. eCollection 2020.
3
Induction of plasmid-mediated AmpC β-lactamase DHA-1 by piperacillin/tazobactam and other β-lactams in Enterobacteriaceae.诱导质粒介导的 AmpC β-内酰胺酶 DHA-1 由哌拉西林/他唑巴坦和其他β-内酰胺类药物在肠杆菌科。
PLoS One. 2019 Jul 8;14(7):e0218589. doi: 10.1371/journal.pone.0218589. eCollection 2019.
4
Probing the Mechanism of Inactivation of the FOX-4 Cephamycinase by Avibactam.探究阿维巴坦对 FOX-4 头孢菌素酶失活机制。
Antimicrob Agents Chemother. 2018 Apr 26;62(5). doi: 10.1128/AAC.02371-17. Print 2018 May.
5
Distribution and Classification of Serine β-Lactamases in Brazilian Hospital Sewage and Other Environmental Metagenomes Deposited in Public Databases.巴西医院污水及公共数据库中其他环境宏基因组中丝氨酸β-内酰胺酶的分布与分类
Front Microbiol. 2016 Nov 15;7:1790. doi: 10.3389/fmicb.2016.01790. eCollection 2016.
6
Comparative genomics of an IncA/C multidrug resistance plasmid from Escherichia coli and Klebsiella isolates from intensive care unit patients and the utility of whole-genome sequencing in health care settings.来自重症监护病房患者的大肠杆菌和克雷伯菌分离株中IncA/C多药耐药质粒的比较基因组学以及全基因组测序在医疗环境中的应用
Antimicrob Agents Chemother. 2014 Aug;58(8):4814-25. doi: 10.1128/AAC.02573-14. Epub 2014 Jun 9.
7
A kinetic analysis of the inhibition of FOX-4 β-lactamase, a plasmid-mediated AmpC cephalosporinase, by monocyclic β-lactams and carbapenems.单环β-内酰胺类和碳青霉烯类对质粒介导的AmpC头孢菌素酶FOX-4β-内酰胺酶抑制作用的动力学分析
J Antimicrob Chemother. 2014 Mar;69(3):682-90. doi: 10.1093/jac/dkt434. Epub 2013 Nov 13.
8
Characterization of the new AmpC β-lactamase FOX-8 reveals a single mutation, Phe313Leu, located in the R2 loop that affects ceftazidime hydrolysis.新型 FOX-8 AmpCβ-内酰胺酶的特性研究表明，位于 R2 环的单个突变 Phe313Leu 影响头孢他啶的水解。
Antimicrob Agents Chemother. 2013 Oct;57(10):5158-61. doi: 10.1128/AAC.00818-13. Epub 2013 Jul 22.
9
10
VIM-15 and VIM-16, two new VIM-2-like metallo-beta-lactamases in Pseudomonas aeruginosa isolates from Bulgaria and Germany.VIM-15和VIM-16，从保加利亚和德国分离出的铜绿假单胞菌中两种新的类VIM-2金属β-内酰胺酶。
Antimicrob Agents Chemother. 2008 Aug;52(8):2977-9. doi: 10.1128/AAC.00175-08. Epub 2008 Jun 2.
本文引用的文献
1
Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC beta-lactamase, and the foss of an outer membrane protein.肺炎克雷伯菌对亚胺培南的耐药性与质粒介导的AmpCβ-内酰胺酶ACT-1和外膜蛋白缺失的联合作用有关。
Antimicrob Agents Chemother. 1997 Mar;41(3):563-9. doi: 10.1128/AAC.41.3.563.
2
Comparative characterization of the cephamycinase blaCMY-1 gene and its relationship with other beta-lactamase genes.头孢菌素酶blaCMY-1基因的比较特征及其与其他β-内酰胺酶基因的关系。
Antimicrob Agents Chemother. 1996 Aug;40(8):1926-30. doi: 10.1128/AAC.40.8.1926.
3
Characterization of the plasmidic beta-lactamase CMY-2, which is responsible for cephamycin resistance.负责头孢霉素耐药性的质粒β-内酰胺酶CMY-2的特性分析。
Antimicrob Agents Chemother. 1996 Jan;40(1):221-4. doi: 10.1128/AAC.40.1.221.
4
Identification of a novel plasmid-mediated beta-lactamase with chromosomal cephalosporinase characteristics from Klebsiella pneumoniae.从肺炎克雷伯菌中鉴定出一种具有染色体头孢菌素酶特性的新型质粒介导的β-内酰胺酶。
J Antimicrob Chemother. 1993 May;31(5):645-54. doi: 10.1093/jac/31.5.645.
5
Characterization of a plasmid-borne and constitutively expressed blaMOX-1 gene encoding AmpC-type beta-lactamase.编码AmpC型β-内酰胺酶的质粒携带型组成型表达blaMOX-1基因的特性分析
Gene. 1994 Feb 11;139(1):93-8. doi: 10.1016/0378-1119(94)90529-0.
6
Cloning and sequence analysis of blaBIL-1, a plasmid-mediated class C beta-lactamase gene in Escherichia coli BS.大肠杆菌BS中质粒介导的C类β-内酰胺酶基因blaBIL-1的克隆与序列分析
Antimicrob Agents Chemother. 1994 May;38(5):1182-5. doi: 10.1128/AAC.38.5.1182.
7
Gene sequence and biochemical characterization of FOX-1 from Klebsiella pneumoniae, a new AmpC-type plasmid-mediated beta-lactamase with two molecular variants.肺炎克雷伯菌中FOX-1的基因序列和生化特性，一种具有两种分子变体的新型AmpC型质粒介导的β-内酰胺酶。
Antimicrob Agents Chemother. 1994 Sep;38(9):2150-7. doi: 10.1128/AAC.38.9.2150.
8
A functional classification scheme for beta-lactamases and its correlation with molecular structure.β-内酰胺酶的功能分类方案及其与分子结构的相关性。
Antimicrob Agents Chemother. 1995 Jun;39(6):1211-33. doi: 10.1128/AAC.39.6.1211.
9
ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.大肠杆菌K-12的AmpC头孢菌素酶与青霉素酶型β-内酰胺酶有着不同的进化起源。
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4897-901. doi: 10.1073/pnas.78.8.4897.
10
Sequence of the Citrobacter freundii OS60 chromosomal ampC beta-lactamase gene.弗氏柠檬酸杆菌OS60染色体ampCβ-内酰胺酶基因序列
Eur J Biochem. 1986 May 2;156(3):441-5. doi: 10.1111/j.1432-1033.1986.tb09601.x.
Zotero 插件