Sth132I，嗜热链球菌ST132的一种新型IIS类限制性内切酶。
Sth132I, a novel class-IIS restriction endonuclease of Streptococcus thermophilus ST132.
作者信息
Poch M T, Somkuti G A, Solaiman D K
机构信息
U.S. Department of Agriculture, ARS, Eastern Regional Research Center, Wyndmoor, PA 19038, USA.
出版信息
Gene. 1997 Aug 22;195(2):201-6. doi: 10.1016/s0378-1119(97)00153-4.
The Sth132I restriction endonuclease (R.Sth132I) was detected in Streptococcus thermophilus ST132 and purified to near homogeneity by heparin Sepharose CL-6B affinity chromatography. Fragments from Sth132I digestion of plasmid DNA were subcloned into pUC19 in Escherichia coli DH5alpha and sequenced. Sequence analysis of inserts and their ligation junction sites revealed that Sth132I is a novel class-IIS restriction endonuclease, which recognizes the non-palindromic sequence 5'-CCCG(N)4-3', 3'-GGGC(N) 8-5'.
嗜热链球菌ST132中检测到Sth132I限制性内切酶（R.Sth132I），并通过肝素琼脂糖CL-6B亲和层析纯化至接近均一。质粒DNA经Sth132I消化产生的片段在大肠杆菌DH5α中被亚克隆到pUC19中并进行测序。对插入片段及其连接位点的序列分析表明，Sth132I是一种新型的IIS类限制性内切酶，其识别非回文序列5'-CCCG(N)4-3'，3'-GGGC(N)8-5'。
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