唾液链球菌嗜热亚种新型II型限制性内切酶SslI的纯化、性质及序列特异性
Purification, properties, and sequence specificity of SslI, a new type II restriction endonuclease from Streptococcus salivarius subsp. thermophilus.
作者信息
Benbadis L, Garel J R, Hartley D L
机构信息
Centre International de Recherche Daniel Carasso, Le Plessis Robinson, France.
出版信息
Appl Environ Microbiol. 1991 Dec;57(12):3677-8. doi: 10.1128/aem.57.12.3677-3678.1991.
Abstract
SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated.
摘要
SslI是一种II型限制性内切酶，从嗜热唾液链球菌亚种嗜热栖热菌菌株BSN 45中纯化得到。SslI是BstNI的同裂酶。SslI的活性在pH 8.8、0至50 mM氯化钠、2至8 mM镁离子以及42摄氏度时达到最大值。已证明其在体外对噬菌体DNA具有活性。
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