Furkert J, Klug U, Slominski A, Eichmüller S, Mehlis B, Kertscher U, Paus R
Research Institute for Molecular Pharmacology, Berlin, Germany.
Biochim Biophys Acta. 1997 Aug 29;1336(2):315-22. doi: 10.1016/s0304-4165(97)00046-9.
We describe new and effective techniques for extracting proopiomelanocortin (POMC)-derived peptides from mammaliar skin. Using this methodology (hot-acid extraction) and two independent HPLC-controlled RIA systems, we identify beta-endorphin peptide in mammalian skin and demonstrate significant hair cycle-dependent fluctuations in both the skin concentration and the in situ expression pattern of beta-endorphin (sebaceous glands) during the entire murine hair cycle. The observed anagen (growth phase) associated increase in beta-endorphin concentration and its decline during the follicle involution (catagen) or resting (telogen) phase raise the possibility of a regulatory function of this neuropeptide in cyclic changes of skin physiology.
我们描述了从哺乳动物皮肤中提取阿片促黑激素原（POMC）衍生肽的新有效技术。使用这种方法（热酸提取）和两个独立的HPLC控制的放射免疫分析系统，我们在哺乳动物皮肤中鉴定出β-内啡肽，并证明在整个小鼠毛发周期中，β-内啡肽（皮脂腺）的皮肤浓度和原位表达模式存在显著的毛发周期依赖性波动。观察到生长期（生长阶段）β-内啡肽浓度增加，而在毛囊退化期（退行期）或静止期（休止期）下降，这增加了这种神经肽在皮肤生理循环变化中具有调节功能的可能性。
