Loesch A, Turmaine M, Loizidou M, Crowe R, Ashraf S, Taylor I, Burnstock G
Department of Anatomy and Developmental Biology, University College London, UK.
J Anat. 1997 Aug;191 ( Pt 2)(Pt 2):291-9. doi: 10.1046/j.1469-7580.1997.19120291.x.
Using electron immunocytochemistry, blood vessels in the normal rat liver and in 2 different animal models of liver metastases: (1) Hooded Lister rat with MC28 tumour, a sarcoma, and (2) nude rat with HT29 tumour, a carcinoma, were investigated for the presence of endothelin-1. In the normal livers, small subpopulations of vascular endothelial cells displayed discrete immunoreactivity for endothelin-1. In the livers with malignant tumours, there was a substantial increase in endothelin-1-immunoreactive endothelial cells in vessels located at the tumour periphery. In the controls, antibody to endothelin-1 also labelled sporadically some fibroblast/fibroblast-like cells associated with the blood vessels. In contrast, intense immunoreactivity for endothelin-1 was frequently associated with the tumour cells and/or fibroblast cells in both types of tumour examined.
利用电子免疫细胞化学技术，对正常大鼠肝脏以及两种不同肝转移动物模型中的血管进行了研究，以检测内皮素-1的存在情况。这两种动物模型分别为：（1）携带MC28肿瘤（一种肉瘤）的带帽利斯特大鼠；（2）携带HT29肿瘤（一种癌）的裸鼠。在正常肝脏中，小部分血管内皮细胞显示出对内皮素-1的离散免疫反应性。在患有恶性肿瘤的肝脏中，位于肿瘤周边血管内的内皮素-1免疫反应性内皮细胞大幅增加。在对照组中，内皮素-1抗体也偶尔标记一些与血管相关的成纤维细胞/成纤维细胞样细胞。相比之下，在所检查的两种肿瘤类型中，内皮素-1的强烈免疫反应性常常与肿瘤细胞和/或成纤维细胞相关。
