Ide N, Lau B H
Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, CA 92350, USA.
J Pharm Pharmacol. 1997 Sep;49(9):908-11. doi: 10.1111/j.2042-7158.1997.tb06134.x.
Oxidation of low density lipoprotein (LDL) has been recognized as playing an important role in the initiation and progression of atherosclerosis. In this study, the effects of aged garlic extract and one of its major compounds, S-allylcysteine, on oxidized LDL-induced cell injury were studied. Pulmonary artery endothelial cells were pre-incubated with the garlic extract (1, 2.5 and 5 mg mL-1) or S-allylcysteine (0.1, 1, 10 and 20 mM) at 37 degrees C and 5% CO2 for 24 h, washed, and then exposed to 0.1 mg mL-1 oxidized LDL for 24 h. Lactate dehydrogenase release as an index of membrane damage, methylthiazol tetrazolium assay for cell viability and thiobarbituric acid reactive substances indicating lipid peroxidation were determined. Preincubation of endothelial cells with the extract or S-allylcysteine significantly prevented membrane damage, loss of cell viability and lipid peroxidation. The data indicate that these compounds can protect vascular endothelial cells from injury caused by oxidized LDL, and suggest that they may be useful for prevention of atherosclerosis.
低密度脂蛋白（LDL）的氧化被认为在动脉粥样硬化的发生和发展中起重要作用。在本研究中，研究了 aged garlic extract 及其主要化合物之一 S-烯丙基半胱氨酸对氧化型 LDL 诱导的细胞损伤的影响。肺动脉内皮细胞在 37℃、5%二氧化碳条件下，用大蒜提取物（1、2.5 和 5mg/mL）或 S-烯丙基半胱氨酸（0.1、1、10 和 20mM）预孵育 24 小时，洗涤后，再暴露于 0.1mg/mL 氧化型 LDL 中 24 小时。测定乳酸脱氢酶释放作为膜损伤指标、用甲基噻唑四氮唑法检测细胞活力以及用硫代巴比妥酸反应性物质检测脂质过氧化情况。内皮细胞与提取物或 S-烯丙基半胱氨酸预孵育可显著预防膜损伤、细胞活力丧失和脂质过氧化。数据表明这些化合物可保护血管内皮细胞免受氧化型 LDL 引起的损伤，并提示它们可能对预防动脉粥样硬化有用。
