Tada T, Matsumoto K, Suzuki H
Second Department of Surgery, Mie University School of Medicine, Tsu-City, Japan.
Surg Today. 1997;27(6):506-10. doi: 10.1007/BF02385803.
Electrochemotherapy is a novel antitumor treatment involving the systemic administration of bleomycin followed by the delivery of electrical pulses to the tumor. The present study investigates the effects of electrochemotherapy on the growth of colon 26 cells inoculated subcutaneously into the backs of BALB/c mice. The mice were divided into the following four experimental groups: 20 that received no further treatment after the inoculation of colon 26 cells (control group); 20 that received 500 micrograms of bleomycin intraperitoneally 7 and 9 days after the inoculation (BLM group); 20 that received electric pulses to the tumor 7 and 9 days after the inoculation (EP group); and 30 that received electrochemotherapy 7 and 9 days after the inoculation (ECT group). During 28 days of observation, no deaths due to tumor progression occurred in the ECT group, but there were 18 in the control group, 11 in the BLM group, and 18 in the EP group. While weight loss was observed in all groups, it was most remarkable in the control group. Tumor growth was significantly inhibited in the ECT group, compared to the other experimental groups (P < 0.01). The results of this study demonstrated that electrochemotherapy significantly inhibited the growth of colon 26 tumors in mice, without causing any remarkable adverse effects.
电化学疗法是一种新型抗肿瘤治疗方法，包括全身给予博来霉素，随后对肿瘤施加电脉冲。本研究调查了电化学疗法对皮下接种于BALB/c小鼠背部的结肠26细胞生长的影响。小鼠被分为以下四个实验组：20只在接种结肠26细胞后未接受进一步治疗（对照组）；20只在接种后第7天和第9天腹腔注射500微克博来霉素（BLM组）；20只在接种后第7天和第9天对肿瘤施加电脉冲（EP组）；30只在接种后第7天和第9天接受电化学疗法（ECT组）。在28天的观察期内，ECT组未出现因肿瘤进展导致的死亡，但对照组有18只死亡，BLM组有11只死亡，EP组有18只死亡。虽然所有组均观察到体重减轻，但对照组最为明显。与其他实验组相比，ECT组的肿瘤生长受到显著抑制（P < 0.01）。本研究结果表明，电化学疗法可显著抑制小鼠结肠26肿瘤的生长，且未引起任何明显的不良反应。
