Belehradek M, Domenge C, Luboinski B, Orlowski S, Belehradek J, Mir L M
Department of Head and Neck Oncology, Institut Gustave-Roussy, Villejuif, France.
Cancer. 1993 Dec 15;72(12):3694-700. doi: 10.1002/1097-0142(19931215)72:12<3694::aid-cncr2820721222>3.0.co;2-2.
Electrochemotherapy is a new antitumor treatment consisting of electrical pulses administered to the tumor several minutes after intravenous injection of bleomycin. In mice, important antitumor effects were observed on subcutaneously transplanted tumors and on spontaneously occurring mammary carcinomas. Cures were obtained after one single treatment combining bleomycin and electric pulses. In humans, permeation nodules seemed an adequate oncologic situation to assay this new procedure. The authors report the first Phase I-II trial of electrochemotherapy.
Eight patients with 40 permeation nodules of head and neck squamous cell carcinomas were treated with 10 mg/m2 bleomycin intravenous bolus, followed by four or eight short (100 microseconds) and intense (1300 V/cm) pulses administered through two external electrodes located on each side of the treated nodule.
An instantaneous painless contraction of the underlying muscles was regularly observed. Neither local nor general side effects were observed, and electrochemotherapy was well tolerated. In addition, a clear local antitumor efficacy was found: 23 (57%) nodules were in clinical complete response within a few days.
The absence of toxicity, the good tolerance by the patients, and the net antitumor effects observed are encouraging for additional electrochemotherapy developments in clinical oncology.
电化学疗法是一种新型抗肿瘤治疗方法，在静脉注射博来霉素几分钟后，对肿瘤施加电脉冲。在小鼠身上，对皮下移植肿瘤和自发性乳腺癌观察到了重要的抗肿瘤效果。单次联合使用博来霉素和电脉冲治疗后实现了治愈。在人类中，渗透结节似乎是检测这一新技术的合适肿瘤情况。作者报告了电化学疗法的首个I-II期试验。
8例患有40个头颈部鳞状细胞癌渗透结节的患者接受了10mg/m²博来霉素静脉推注治疗，随后通过位于治疗结节两侧的两个外部电极施加四个或八个短（100微秒）而强（1300V/cm）的脉冲。
经常观察到深层肌肉瞬间无痛收缩。未观察到局部或全身副作用，电化学疗法耐受性良好。此外，发现了明确的局部抗肿瘤疗效：23个（57%）结节在几天内达到临床完全缓解。
无毒性、患者耐受性良好以及观察到明显的抗肿瘤效果，这些对于临床肿瘤学中进一步开展电化学疗法是令人鼓舞的。
