Smith P B, Snyder A P, Harden C S
Geo-Centers, Gunpowder Branch, Aberdeen Proving Ground, Maryland 21010-0068, USA.
Anal Chem. 1995 Jun 1;67(11):1824-30. doi: 10.1021/ac00107a011.
A negative ion electrospray ionization tandem mass spectrometric technique was developed for the analysis of glycerophospholipids. Examination of the product ion mass spectrum of the deprotonated molecular ion provided sufficient information to identify both the class of glycerophospholipid and the molecular weights of the two fatty acid moieties. This technique was applied to the profiling of glycerophospholipids present in the chloroform/methanol extracts of four different bacterial species. The principal bacterial phospholipids detected by this technique were phosphatidylglycerols and diphosphatidylglycerols, accompanied by small amounts of phosphatidylethanolamines for two of the bacterial species examined. The fatty acid composition of the phosphatidylglycerols for each bacteria was determined by tandem mass spectrometry and presented graphically. Differences in the fatty acid composition for each bacterial species were readily apparent from a visual examination of the data sets.
开发了一种负离子电喷雾电离串联质谱技术用于甘油磷脂的分析。对去质子化分子离子的产物离子质谱进行检查，可提供足够信息以鉴定甘油磷脂的类别以及两个脂肪酸部分的分子量。该技术应用于四种不同细菌物种氯仿/甲醇提取物中甘油磷脂的分析。通过该技术检测到的主要细菌磷脂是磷脂酰甘油和二磷脂酰甘油，在所检测的两种细菌物种中还伴有少量的磷脂酰乙醇胺。通过串联质谱法测定了每种细菌的磷脂酰甘油的脂肪酸组成，并以图形方式呈现。通过直观检查数据集，每种细菌物种的脂肪酸组成差异显而易见。
