Hampson V, Liu D, Billett E, Kirk S
Department of Life Sciences, Nottingham Trent University, UK.
Br J Obstet Gynaecol. 1997 Sep;104(9):1087-91. doi: 10.1111/j.1471-0528.1997.tb12073.x.
Amniotic membrane samples were taken following preterm delivery from 15 women with preterm prelabour rupture of the membranes (PPROM) and 14 with unrelated reasons, and from 25 delivering at term. Fibrillar collagens were extracted and analysed by interrupted gel electrophoresis. Collagens were decreased in PPROM relative to controls, but showed similar sub-type distribution, with one exception. In this case an increased ratio of Type I to Type III collagen was observed, with normal collagen content. In conclusion, PPROM is accompanied by generalised reduction in amnion collagen content, involving all fibrillar sub-types monitored. This may reflect general changes in protease activity or in collagen expression in affected membranes.
从15例胎膜早破（PPROM）的早产妇女、14例因其他无关原因早产的妇女以及25例足月分娩的妇女中获取羊膜样本。提取纤维状胶原蛋白并通过间断凝胶电泳进行分析。与对照组相比，PPROM组的胶原蛋白减少，但亚型分布相似，只有一个例外。在这种情况下，观察到I型与III型胶原蛋白的比例增加，而胶原蛋白含量正常。总之，PPROM伴有羊膜胶原蛋白含量的普遍降低，涉及所有监测的纤维状亚型。这可能反映了受影响胎膜中蛋白酶活性或胶原蛋白表达的总体变化。
