Weeks R A, Cunningham V J, Piccini P, Waters S, Harding A E, Brooks D J
MRC Cyclotron Unit, Hammersmith Hospital, London, United Kingdom.
J Cereb Blood Flow Metab. 1997 Sep;17(9):943-9. doi: 10.1097/00004647-199709000-00003.
We compare region of interest (ROI) analytical approaches with statistical parametric mapping (SPM) of 11C-diprenorphine positron emission tomography findings in five patients with Huntington's disease (HD) and nine age-matched controls. The ROI were placed on caudate, putamen, and an occipital reference area. Ratios of striatal-occipital uptake from averaged static images centered at 60 minutes showed a mean 20% reduction in caudate (P = 0.034) and 15% reduction in putamen (P = 0.095) receptor binding in the HD patients. Dynamic data from caudate and putamen ROI, together with a plasma tracer input function, were analyzed using spectral analysis to give regional impulse response functions. Regional data at 60 minutes after impulse showed a mean 29% decrease in caudate (P = 0.006) and 23% decrease in putamen (P = 0.029) opioid binding in the HD cohort. Parametric images of tracer binding also were produced with spectral analysis on a voxel basis. The images of the unit impulse response function at 60 minutes showed a mean 31% decrease in caudate (P = 0.005) and a 26% decrease in putamen binding (P = 0.011) in HD. The voxel-based parametric images were transformed into standard stereotactic space, and a between-group comparison (patient versus controls) was performed with SPM. This approach revealed symmetrical decreases in caudate (peak 40% decrease, z score = 4.38) and putamen opioid binding (peak 24% decrease, z score = 4.686) with additional nonhypothesized changes in cingulate, prefrontal, and thalamic areas. The significance and precision of changes measured with spectral analysis applied to dynamic data sets were superior to ROI-based ratio analysis on static images. The SPM replicated the striatal reductions in opioid binding in HD and detected additional nonpredicted changes. This study suggests that SPM is a valid alternative to conventional ROI analytical approaches for determining binding changes with positron emission tomography and may have advantages over region-based analyses in exploratory studies.
我们将感兴趣区域（ROI）分析方法与11C-二丙诺啡正电子发射断层扫描结果的统计参数映射（SPM）进行了比较，研究对象为5例亨廷顿舞蹈病（HD）患者和9名年龄匹配的对照者。将ROI置于尾状核、壳核和枕叶参考区域。以60分钟为中心的平均静态图像的纹状体-枕叶摄取率显示，HD患者的尾状核受体结合平均降低20%（P = 0.034），壳核降低15%（P = 0.095）。使用频谱分析对来自尾状核和壳核ROI的动态数据以及血浆示踪剂输入函数进行分析，以得出区域脉冲响应函数。脉冲后60分钟的区域数据显示，HD队列中尾状核的阿片样物质结合平均降低29%（P = 0.006），壳核降低23%（P = 0.029）。还通过基于体素的频谱分析生成了示踪剂结合的参数图像。60分钟时单位脉冲响应函数的图像显示，HD患者尾状核结合平均降低31%（P = 0.005），壳核结合降低26%（P = 0.011）。基于体素的参数图像被转换为标准立体定向空间，并使用SPM进行组间比较（患者与对照）。该方法显示尾状核（峰值降低40%，z值 = 4.38）和壳核阿片样物质结合（峰值降低24%，z值 = 4.686）出现对称性降低，同时扣带回、前额叶和丘脑区域出现额外的非假设性变化。应用于动态数据集的频谱分析所测量变化的显著性和精确性优于基于静态图像的ROI比率分析。SPM复制了HD患者纹状体中阿片样物质结合的降低情况，并检测到其他未预测到的变化。本研究表明，在通过正电子发射断层扫描确定结合变化方面，SPM是传统ROI分析方法的有效替代方法，并且在探索性研究中可能比基于区域的分析具有优势。
