Halvorsen D S, Hansen J B, Grimsgaard S, Bønaa K H, Kierulf P, Nordøy A
Department of Medicine, University of Tromsø, Norway.
Lipids. 1997 Sep;32(9):935-42. doi: 10.1007/s11745-997-0120-2.
The n-3 fatty acids (FA) from marine sources are known to exert antiinflammatory effects on monocyte function. There is still controversy whether n-3 FA may increase the susceptibility to infections. The present study was designed to assess the effect of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on monocyte phagocytosis and respiratory burst activity. Fifty-eight healthy men were randomized to take a daily supplement of 3.8 g highly purified EPA (n = 20), 3.6 g DHA (n = 19), or corn oil (n = 19) for 7 wk. Mononuclear leukocytes were collected, isolated, and cryopreserved prior to and after dietary supplementation. Paired samples were analyzed in the presence of autologous serum in a crossover design. Monocyte phagocytosis and respiratory burst activity were measured by flow cytometry after ingestion of Escherichia coli. Monocytes retained their phagocytic ability and respiratory burst activity after supplementation. No reduction in internalization of bacteria was registered. Dietary n-3 FA and particularly EPA improved bacterial adherence to the monocyte surface. In the crossover experiments, there was an adverse effect of serum enriched with n-3 FA on bacterial adherence. We conclude that monocytes retain their phagocytic potential after supplementation with purified EPA and DHA.
已知海洋来源的n-3脂肪酸（FA）对单核细胞功能具有抗炎作用。n-3 FA是否会增加感染易感性仍存在争议。本研究旨在评估二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）对单核细胞吞噬作用和呼吸爆发活性的影响。58名健康男性被随机分为三组，分别每日补充3.8 g高纯度EPA（n = 20）、3.6 g DHA（n = 19）或玉米油（n = 19），为期7周。在饮食补充前后收集、分离并冷冻保存单核白细胞。采用交叉设计在自体血清存在的情况下分析配对样本。摄入大肠杆菌后，通过流式细胞术测量单核细胞吞噬作用和呼吸爆发活性。补充后单核细胞保留了其吞噬能力和呼吸爆发活性。未发现细菌内化减少。饮食中的n-3 FA，尤其是EPA，改善了细菌对单核细胞表面的黏附。在交叉实验中，富含n-3 FA的血清对细菌黏附有不利影响。我们得出结论，补充纯化的EPA和DHA后，单核细胞保留了其吞噬潜力。
