Rpp1是酿酒酵母中前体tRNA和35S前体rRNA加工所需的核核糖核酸酶P的一种必需蛋白质亚基。
Rpp1, an essential protein subunit of nuclear RNase P required for processing of precursor tRNA and 35S precursor rRNA in Saccharomyces cerevisiae.
作者信息
Stolc V, Altman S
机构信息
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
出版信息
Genes Dev. 1997 Sep 15;11(18):2414-25. doi: 10.1101/gad.11.18.2414.
Abstract
The gene for an essential protein subunit of nuclear RNase P from Saccharomyces cerevisiae has been cloned. The gene for this protein, RPP1, was identified by virtue of its homology with a human scleroderma autoimmune antigen, Rpp30, which copurifies with human RNase P. Epitope-tagged Rpp1 can be found in association with both RNase P RNA and a related endoribonuclease, RNase MRP RNA, in immunoprecipitates from crude extracts of cells. Depletion of Rpp1 in vivo leads to the accumulation of precursor tRNAs with unprocessed 5' and 3' termini and reveals rRNA processing defects that have not been described previously for proteins associated with RNase P or RNase MRP. Immunoprecipitated complexes cleave both yeast precursor tRNAs and precursor rRNAs.
摘要
酿酒酵母核核糖核酸酶P的一种必需蛋白质亚基的基因已被克隆。该蛋白质的基因RPP1是通过与人类硬皮病自身免疫抗原Rpp30的同源性鉴定出来的，Rpp30与人类核糖核酸酶P共纯化。在细胞粗提物的免疫沉淀中，可发现表位标记的Rpp1与核糖核酸酶P RNA和一种相关的核糖核酸内切酶核糖核酸酶MRP RNA结合。体内Rpp1的缺失导致具有未加工5'和3'末端的前体tRNA积累，并揭示了与核糖核酸酶P或核糖核酸酶MRP相关蛋白质以前未描述过的rRNA加工缺陷。免疫沉淀复合物可切割酵母前体tRNA和前体rRNA。
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