使用电阻抗细胞计数器检测微血小板形成。
Detection of microplatelet formation using an electroimpedance cell counter.
作者信息
Kawasaka K, Takubo T, Takekawa K, Oota T, Kohl K R, Hashimoto S, Hirai N, Tsuda I, Tatsumi N
机构信息
Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Japan.
出版信息
Osaka City Med J. 1994 Jul;40(1):13-8.
Changes in the platelet size distribution curves during the process of ADP-induced aggregation were observed using a particle distribution analyzer (PDA) based on electronic impedance. The maximum primary aggregation was seen at a final ADP concentration of 3 microM. After stimulation, the platelet distribution curve changed rapidly from a log normal curve to a curve skewed to the left. When the changes were evaluated at 1 min after the addition of ADP, the peak height was increased, and the median and total volume were decreased significantly. These findings indicate that microplatelets are formed by membrane stimulation and this formation can be monitored with the use of this automated instrument.
使用基于电阻抗的颗粒分布分析仪（PDA）观察了ADP诱导聚集过程中血小板大小分布曲线的变化。在最终ADP浓度为3 microM时观察到最大的初始聚集。刺激后，血小板分布曲线迅速从对数正态曲线变为向左偏斜的曲线。当在添加ADP后1分钟评估变化时，峰高增加，中位数和总体积显著降低。这些发现表明，微血小板是通过膜刺激形成的，并且可以使用这种自动化仪器对这种形成进行监测。
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