Jarrett W, Jarrett O, Mackey L, Laird H, Hood C, Hay D
Int J Cancer. 1975 Jul 15;16(1):134-41. doi: 10.1002/ijc.2910160115.
Cats inoculated with live feline lymphoblastoid cells of the FL74 line developed high titres of antibody to feline oncornavirus-associated cell membrane antigen (FOCMA). Eight cats were subsequently challenged with a large dose of feline leukaemia virus (FeLV) of a highly pathogenic strain. All resisted infection while 10 cats given the challenge virus alone became infected. The FeLV produced by FL74 cells was shown to be of extremely low infectivity in cats and in cultured feline cells. Cats inoculated with either FL74 cells or virus purified from them did not become infected. The purified virus did not induce FOCMA antibody in cats not previously exposed to FeLV. The fact that FL74 cells are highly immunogenic, but produce virus of low infectivity, is of value in devising vaccines against FeLV. Cats were also inoculated with FL74 cells which had been inactivated with paraformaldehyde. They developed FOCMA antibody, reaching a peak titre of 256, and no virus could be cultured either from the vaccine preparations or from the tissues of the cats.
用FL74系的活猫淋巴母细胞接种的猫，产生了高滴度的抗猫肿瘤病毒相关细胞膜抗原（FOCMA）抗体。随后，用大剂量高致病性毒株的猫白血病病毒（FeLV）对8只猫进行攻击。所有猫都抵抗了感染，而仅接受攻击病毒的10只猫被感染。结果表明，FL74细胞产生的FeLV在猫和培养的猫细胞中的感染性极低。接种FL74细胞或从其中纯化的病毒的猫没有被感染。纯化的病毒在未接触过FeLV的猫中不会诱导FOCMA抗体。FL74细胞具有高度免疫原性，但产生的病毒感染性低，这一事实在设计抗FeLV疫苗方面具有价值。还用经多聚甲醛灭活的FL74细胞接种猫。它们产生了FOCMA抗体，峰值滴度达到256，并且从疫苗制剂或猫的组织中均未培养出病毒。
