Ishiyama H, Ogino K, Sato M, Ogura M, Dan S, Hobara T
Department of Public Health, Yamaguchi University School of Medicine, Ube, Japan.
Exp Toxicol Pathol. 1997 Aug;49(3-4):261-6. doi: 10.1016/S0940-2993(97)80027-9.
Rat lungs were histologically examined at 1, 7, 14 and 28 days following a single intratracheal instillation of zinc hydroxide (1 mM). After one day of treatment, no confirmatory findings were noted. The zinc hydroxide injections were followed by an increase in proliferating cell nuclear antigen labeling indices in both alveolar macrophages and terminal bronchioles. After 7 days, the zinc hydroxide-treated lungs showed thickening of the interstitium with infiltration by alveolar macrophages, and an increase in the grade of Masson's trichrome staining (collagen fiber) in the alveolar interstitium. Thereafter, these morphological changes disappeared. The vehicle- and zinc sulfate (1 mM)-exposed lungs had no abnormalities at any time point. Formazan deposits in alveolar macrophages, formed as a result of nitro blue tetrazolium reduction, were increased in zinc hydroxide-treated lung slices, suggesting that zinc hydroxide stimulated super oxide anion generation from alveolar macrophages. These results show that zinc hydroxide can induce morphological alterations of rat lungs.
在单次气管内注入氢氧化锌（1 mM）后的第1、7、14和28天，对大鼠肺部进行组织学检查。治疗一天后，未观察到确诊结果。注射氢氧化锌后，肺泡巨噬细胞和终末细支气管中的增殖细胞核抗原标记指数增加。7天后，用氢氧化锌处理的肺部显示间质增厚，伴有肺泡巨噬细胞浸润，肺泡间质中马松三色染色（胶原纤维）等级增加。此后，这些形态学变化消失。在任何时间点，接受赋形剂和硫酸锌（1 mM）处理的肺部均无异常。在氢氧化锌处理的肺切片中，由于硝基蓝四氮唑还原而在肺泡巨噬细胞中形成的甲臜沉积物增加，这表明氢氧化锌刺激了肺泡巨噬细胞产生超氧阴离子。这些结果表明，氢氧化锌可诱导大鼠肺部的形态学改变。
