Ogino K, Izumi Y, Ishiyama H, Murata T, Kobayashi H, Houbara T
Department of Public Health, Yamaguchi University School of Medicine, Ube, Japan.
Biochem Biophys Res Commun. 1992 Jun 30;185(3):1115-21. doi: 10.1016/0006-291x(92)91741-8.
The effect of zinc hydroxide on superoxide (O2-) production by rat alveolar macrophages was determined by chemiluminescence and by cytochrome c reduction. Zinc ions had no effect on the chemiluminescence of unstimulated alveolar macrophages. By contrast, zinc hydroxide (ZnOH2), a neutralized form of zinc ions, increased the chemiluminescence level and O2- release. Increased O2- release was inhibited by pertussis toxin, isoquinoline sulfonamide and pretreatment with EGTA. These findings indicate that zinc hydroxide formation from zinc compounds can stimulate the O2- production by alveolar macrophages by receptor-mediated and Ca(2+)-dependent process.
通过化学发光法和细胞色素c还原法测定了氢氧化锌对大鼠肺泡巨噬细胞产生超氧化物（O2-）的影响。锌离子对未受刺激的肺泡巨噬细胞的化学发光没有影响。相比之下，锌离子的中和形式氢氧化锌（ZnOH2）增加了化学发光水平和O2-释放。百日咳毒素、异喹啉磺酰胺和用乙二醇双四乙酸（EGTA）预处理可抑制O2-释放的增加。这些发现表明，锌化合物形成的氢氧化锌可通过受体介导和Ca（2+）依赖的过程刺激肺泡巨噬细胞产生O2-。
