人类CD4 + T细胞克隆产生并释放神经生长因子，并表达高亲和力的神经生长因子受体。

Human CD4+ T cell clones produce and release nerve growth factor and express high-affinity nerve growth factor receptors.

作者信息

Lambiase A, Bracci-Laudiero L, Bonini S, Bonini S, Starace G, D'Elios M M, De Carli M, Aloe L

机构信息

Institute of Neurobiology, Consiglio Nazionale Richerche, Department of Ophthalmology, University of Rome Tor Vergata, Italy.

出版信息

J Allergy Clin Immunol. 1997 Sep;100(3):408-14. doi: 10.1016/s0091-6749(97)70256-2.

DOI:10.1016/s0091-6749(97)70256-2

PMID:9314355

Abstract

BACKGROUND

Increasing evidence shows that nerve growth factor (NGF) plays a role in the complex and fascinating linkage between the nervous and the immune systems due to its ability to modulate functions of several inflammatory cells.

OBJECTIVE

To investigate NGF receptor expression and NGF production and release by human CD4+ cells clones, which have primary relevance in modulating inflammatory events through their different subsets of functional phenotypes.

METHODS

The expression of NGF and a transmembrane tyrosine kinase (TrkA) was evaluated by immunohistochemistry and flow cytometry analysis in five T(H0), six T(H1), and five T(H2) cell clones derived from human circulating mononuclear blood cells. Moreover, the amount of NGF protein was assessed by measuring the NGF levels in culture supernatants of the T cell clones before stimulation and 48 hours after phytohemagglutinin (PHA) activation by use of an immunoenzymatic assay.

RESULTS

Our data have shown that in unstimulated conditions, human CD4+ T cell clones express both immunoreactivity for NGF and the TrkA NGF receptor irrespective of their cytokine profile. Moreover, T(H1) and T(H2) clones, but not T(H0) clones, secrete NGF in basal conditions. PHA activation induces NGF secretion in T(H0) clones and a significant increase of NGF levels in T(H2) (p < 0.05), but not in T(H1) culture supernatants.

CONCLUSIONS

Results obtained represent the first evidence of TrkA expression and NGF production and release in human CD4+ cell clones and suggest a possible functional role of NGF in modulating the immune and inflammatory network.

摘要

背景

越来越多的证据表明，神经生长因子（NGF）由于能够调节多种炎症细胞的功能，在神经和免疫系统之间复杂而迷人的联系中发挥作用。

目的

研究人CD4 +细胞克隆中NGF受体的表达以及NGF的产生和释放，这些克隆通过其不同的功能表型亚群在调节炎症事件中具有主要相关性。

方法

通过免疫组织化学和流式细胞术分析评估了从人循环单核血细胞衍生的五个T（H0）、六个T（H1）和五个T（H2）细胞克隆中NGF和跨膜酪氨酸激酶（TrkA）的表达。此外，通过使用免疫酶测定法测量T细胞克隆在刺激前和植物血凝素（PHA）激活后48小时的培养上清液中的NGF水平，评估NGF蛋白的量。

结果

我们的数据表明，在未刺激的条件下，人CD4 + T细胞克隆无论其细胞因子谱如何，均表达对NGF的免疫反应性和TrkA NGF受体。此外，T（H1）和T（H2）克隆而非T（H0）克隆在基础条件下分泌NGF。PHA激活诱导T（H0）克隆分泌NGF，并使T（H2）培养上清液中的NGF水平显著增加（p < 0.05），但T（H1）培养上清液中未增加。