Du S J, Devoto S H, Westerfield M, Moon R T
Howard Hughes Medical Institute, University of Washington, School of Medicine, Seattle 98195, USA.
J Cell Biol. 1997 Oct 6;139(1):145-56. doi: 10.1083/jcb.139.1.145.
We have examined whether the development of embryonic muscle fiber type is regulated by competing influences between Hedgehog and TGF-beta signals, as previously shown for development of neuronal cell identity in the neural tube. We found that ectopic expression of Hedgehogs or inhibition of protein kinase A in zebrafish embryos induces slow muscle precursors throughout the somite but muscle pioneer cells only in the middle of the somite. Ectopic expression in the notochord of Dorsalin-1, a member of the TGF-beta superfamily, inhibits the formation of muscle pioneer cells, demonstrating that TGF-beta signals can antagonize the induction of muscle pioneer cells by Hedgehog. We propose that a Hedgehog signal first induces the formation of slow muscle precursor cells, and subsequent Hedgehog and TGF-beta signals exert competing positive and negative influences on the development of muscle pioneer cells.
我们研究了胚胎肌纤维类型的发育是否受刺猬信号通路（Hedgehog）和转化生长因子β信号通路（TGF-β）之间竞争性影响的调控，正如先前在神经管中神经元细胞身份发育研究中所显示的那样。我们发现，在斑马鱼胚胎中异位表达刺猬信号蛋白或抑制蛋白激酶A会在整个体节诱导慢肌前体细胞的形成，但仅在体节中部诱导肌肉先驱细胞的形成。转化生长因子β超家族成员背侧素1（Dorsalin-1）在脊索中的异位表达会抑制肌肉先驱细胞的形成，这表明转化生长因子β信号可以拮抗刺猬信号通路对肌肉先驱细胞的诱导作用。我们提出，刺猬信号通路首先诱导慢肌前体细胞的形成，随后刺猬信号通路和转化生长因子β信号通路对肌肉先驱细胞的发育产生竞争性的正向和负向影响。
