Production of antibodies against ML antigen in the xenogeneic host.

By using various protocols of immunization in the xenogeneic system, a serum was produced which contained antibodies against normal as well as leukemic lymphocytes in DBA/2 mice. To obtain specific antibodies capable of recognizing the ML tumor antigen, the best protocol proved to be the one used in group 2, in which immunization with leukemic cells was preceded by immunization of newborn mice with normal lymphoid cells from mice of the DBA/2 f BALB/c/MTV-s-negative strain. The sera contained antibodies capable of identifying the ML antigen in leukemia cells as well as in isolated form.