一种用于检测弓形虫抗体的微孔板酶免疫测定法。
A microplate enzyme-immunoassay for toxoplasma antibody.
作者信息
Voller A, Bidwell D E, Bartlett A, Fleck D G, Perkins M, Oladehin B
出版信息
J Clin Pathol. 1976 Feb;29(2):150-3. doi: 10.1136/jcp.29.2.150.
Abstract
A new test for the detection and measurement of toxoplasma antibody is described. Test sera are reacted with antigen-sensitized wells in micro-haemagglutination plates. Any attached antibody is shown by the addition of an enzyme-labelled antiglobulin followed by assay of the enzyme reaction with its substrate. The test is easy to carry out on a large scale, and there is a positive correlation between the results and dye test and haemagglutination test titres.
摘要
本文描述了一种用于检测和测量弓形虫抗体的新试验。将试验血清与微量血凝板中抗原致敏的孔进行反应。通过加入酶标记的抗球蛋白，然后用其底物检测酶反应，来显示任何附着的抗体。该试验易于大规模开展，且结果与染料试验和血凝试验滴度之间存在正相关。
相似文献
1
A microplate enzyme-immunoassay for toxoplasma antibody.一种用于检测弓形虫抗体的微孔板酶免疫测定法。
J Clin Pathol. 1976 Feb;29(2):150-3. doi: 10.1136/jcp.29.2.150.
2
Comparison of a latex agglutination test with other serological tests for the measurement of antibodies to Toxoplasma gondii.乳胶凝集试验与其他用于检测弓形虫抗体的血清学试验的比较。
J Clin Pathol. 1984 Nov;37(11):1293-7. doi: 10.1136/jcp.37.11.1293.
3
Comparative study of three tests (dye test, indirect haemagglutination test, latex agglutination test) for the detection of antibodies to Toxoplasma gondii in human sera.三种检测人血清中弓形虫抗体的试验（染色试验、间接血凝试验、乳胶凝集试验）的比较研究。
J Clin Pathol. 1982 Feb;35(2):228-32. doi: 10.1136/jcp.35.2.228.
4
A standardized enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to Toxoplasma gondii.一种用于检测弓形虫抗体的标准化酶联免疫吸附测定（ELISA）。
J Immunoassay. 1981;2(1):45-57. doi: 10.1080/01971528108062991.
5
An evaluation of the ToxHA test for the detection of antibodies to Toxoplasma gondii in human serum.用于检测人血清中抗弓形虫抗体的ToxHA试验评估。
J Clin Pathol. 1980 Jul;33(7):644-7. doi: 10.1136/jcp.33.7.644.
6
The haemagglutination test for toxoplasma antibodies.弓形虫抗体血凝试验
J Clin Pathol. 1960 Jul;13(4):331-5. doi: 10.1136/jcp.13.4.331.
7
[Use of an immunoenzymatic test in the serological diagnostics of toxoplasmosis (author's transl)].免疫酶试验在弓形虫病血清学诊断中的应用（作者译）
Quad Sclavo Diagn. 1978 Sep;15(3):402-9.
8
Standardized quantitative enzyme-linked immunoassay for antibodies to Toxoplasma gondii.弓形虫抗体标准化定量酶联免疫测定法
J Clin Microbiol. 1980 Jun;11(6):675-81. doi: 10.1128/jcm.11.6.675-681.1980.
9
ELISA for toxoplasma antibody detection: a comparison with other serodiagnostic tests.用于弓形虫抗体检测的酶联免疫吸附测定（ELISA）：与其他血清学诊断试验的比较
J Clin Pathol. 1980 Jul;33(7):640-3. doi: 10.1136/jcp.33.7.640.
10
Comparison of the indirect haemagglutination and dye test for detection of antibodies to Toxoplasma gondii.间接血凝试验与染色试验检测弓形虫抗体的比较
Scand J Infect Dis. 1974;6(1):63-8. doi: 10.3109/inf.1974.6.issue-1.11.
引用本文的文献
1
Prevalence, knowledge, attitudes, and practices regarding Chagas disease in Guanare, Venezuela: a cross-sectional study.委内瑞拉瓜纳雷地区恰加斯病的患病率、知识、态度及实践：一项横断面研究
Parasit Vectors. 2025 Jun 8;18(1):215. doi: 10.1186/s13071-025-06846-4.
2
Effect of Organic Selenium Supplementation on the Antioxidant Status, Immune Response, and the Relative Expression of IL-2 and IFN-γ Genes in Ewes During the Hot Season.热季节有机硒补充对母羊抗氧化状态、免疫反应和 IL-2 和 IFN-γ 基因相对表达的影响。
Biol Trace Elem Res. 2024 May;202(5):2052-2061. doi: 10.1007/s12011-023-03798-7. Epub 2023 Aug 4.
3
Evaluation of the Tyrosine and Dopamine Serum Levels in Experimental Infected BALB/c Mice with Chronic Toxoplasmosis.实验性感染慢性弓形虫病的BALB/c小鼠血清酪氨酸和多巴胺水平的评估
J Parasitol Res. 2021 Aug 17;2021:5511516. doi: 10.1155/2021/5511516. eCollection 2021.
4
Review on the Current Trends of Toxoplasmosis Serodiagnosis in Humans.人类弓形虫病血清学诊断的当前趋势综述
Front Cell Infect Microbiol. 2020 May 8;10:204. doi: 10.3389/fcimb.2020.00204. eCollection 2020.
5
Beneficial Effects of Honey on the Immune Response of Rats Infected with .蜂蜜对感染……的大鼠免疫反应的有益作用 。
（注：原文中“. ”处内容缺失）
J Pharmacopuncture. 2017 Jun;20(2):112-118. doi: 10.3831/KPI.2017.20.015. Epub 2017 Jun 30.
6
Toxoplasma gondii: Biological Parameters of the Connection to Schizophrenia.刚地弓形虫：与精神分裂症相关的生物学参数。
Schizophr Bull. 2018 Aug 20;44(5):983-992. doi: 10.1093/schbul/sby082.
7
Identification of Toxoplasma gondii antigens associated with different types of infection by serum antibody profiling.通过血清抗体谱分析鉴定与不同类型感染相关的弓形虫抗原。
Parasitology. 2015 May;142(6):827-38. doi: 10.1017/S0031182014001978. Epub 2015 Jan 14.
8
Seroprevalence of Toxoplasma gondii in southern districts of Tamil Nadu using IgG-ELISA.使用IgG-ELISA法检测泰米尔纳德邦南部地区弓形虫的血清阳性率。
J Parasit Dis. 2012 Oct;36(2):159-64. doi: 10.1007/s12639-012-0103-6. Epub 2012 Mar 13.
9
Serological survey of toxoplasmosis in a district in Tamil Nadu: hospital-based study.泰米尔纳德邦某地区弓形虫病的血清学调查：基于医院的研究。
Indian J Med Res. 2013 Mar;137(3):560-3.
10
Identification of potential serodiagnostic and subunit vaccine antigens by antibody profiling of toxoplasmosis cases in Turkey.通过对土耳其弓形虫病病例的抗体谱分析，鉴定潜在的血清学诊断和亚单位疫苗抗原。
Mol Cell Proteomics. 2011 Jul;10(7):M110.006916. doi: 10.1074/mcp.M110.006916. Epub 2011 Apr 21.
本文引用的文献
1
Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma).作为一种影响原生动物寄生虫（弓形虫）的新免疫现象的微化学指示剂的染料。
Science. 1948 Dec 10;108(2815):660-3. doi: 10.1126/science.108.2815.660.
2
A hemagglutination test for toxoplasmosis.一项针对弓形虫病的血凝试验。
J Parasitol. 1957 Jun;43(3):308-14.
3
4
New serological test for malaria antibodies.疟疾抗体新血清学检测方法。
Br Med J. 1975 Mar 22;1(5959):659-61. doi: 10.1136/bmj.1.5959.659.
5
The relationship and significance of antibody titres as determined by various serological methods in glandular and ocular toxoplasmosis.通过各种血清学方法测定的抗体滴度在腺型和眼型弓形虫病中的关系及意义。
J Clin Pathol. 1975 Jan;28(1):42-9. doi: 10.1136/jcp.28.1.42.
6
Microplate enzyme-linked immunosorbent assay for chagas' disease.用于查加斯病的微孔板酶联免疫吸附测定
Lancet. 1975 Feb 22;1(7904):426-8. doi: 10.1016/s0140-6736(75)91492-0.
Zotero 插件