Lin T M, Halbert S P, O'Connor G R
J Clin Microbiol. 1980 Jun;11(6):675-81. doi: 10.1128/jcm.11.6.675-681.1980.
A quantitative and standardized enzyme-linked immunosorbent assay is described which uses lyophilized antigen-coated disks for the detection of human antibodies to Toxoplasma gondii. It does not require serial dilution of the test specimen, and the objective absorbance readings are converted into international units per milliliter traceable to the World Health Organization's reference standard preparation of toxoplasma antibodies. It was shown to be highly specific, reproducible, and sensitive. The reagents were stable, without biohazard, and ready for use. Studies of various parameters in the assay indicated that the test conditions were relatively flexible. The enzyme-linked immunosorbent assay results correlated satisfactorily with the methylene blue dye test, the indirect immunofluorescence test, and the passive hemagglutination test.
描述了一种定量和标准化的酶联免疫吸附测定法,该方法使用冻干的抗原包被圆盘来检测人抗弓形虫抗体。它不需要对测试样本进行系列稀释,并且将客观吸光度读数转换为每毫升国际单位,可追溯至世界卫生组织的弓形虫抗体参考标准制剂。结果表明该方法具有高度特异性、可重复性和敏感性。试剂稳定,无生物危害,可随时使用。该测定法中各种参数的研究表明测试条件相对灵活。酶联免疫吸附测定结果与亚甲蓝染色试验、间接免疫荧光试验和被动血凝试验结果令人满意地相关。