通过片段E放射免疫测定法测量纤维蛋白（原）降解产物时血清样本的采集条件。
Conditions for collection of serum samples for the measurement of fibrin(ogen) degradation products by radioimmunoassay of fragment E.
作者信息
Martin M J, Gordon Y B, Ratky S M, Baker L R, Chard T
出版信息
J Clin Pathol. 1976 Apr;29(4):336-40. doi: 10.1136/jcp.29.4.336.
Abstract
A number of conditions have been assessed for the collection of serum samples for the measurement of fibrin(ogen) degradation products using a radioimmunoassay for degradation fragment E, which permits precise quantitation of differences. Blood should be collected using minimal venous occlusion into glass tubes containing 10 mg/ml of epsilon amino caproic acid and allowed to clot at 4 to 20 degrees C for at least 4 hours before centrifugation. The serum may be stored at 4 or -20 degrees C. Samples from patients receiving anticoagulant therapy should be treated with 10 IU of thrombin per ml.
摘要
已对多种条件进行了评估，以便收集血清样本，使用针对降解片段E的放射免疫测定法来测量纤维蛋白（原）降解产物，该方法可以精确量化差异。应使用最小限度的静脉阻塞将血液采集到含有10mg/mlε-氨基己酸的玻璃管中，并在4至20摄氏度下凝固至少4小时，然后进行离心。血清可储存在4或-20摄氏度。接受抗凝治疗患者的样本应每毫升用10IU凝血酶处理。
相似文献
1
Conditions for collection of serum samples for the measurement of fibrin(ogen) degradation products by radioimmunoassay of fragment E.通过片段E放射免疫测定法测量纤维蛋白（原）降解产物时血清样本的采集条件。
J Clin Pathol. 1976 Apr;29(4):336-40. doi: 10.1136/jcp.29.4.336.
2
Effect of specimen collection on routine coagulation assays and D-dimer measurement.标本采集对常规凝血检测及D-二聚体测定的影响。
Clin Chem. 2004 Nov;50(11):2150-2. doi: 10.1373/clinchem.2004.036244.
3
Identification and distribution of fibrinogen, fibrin, and fibrin(ogen) degradation products in atherosclerosis. Use of monoclonal antibodies.动脉粥样硬化中纤维蛋白原、纤维蛋白及纤维蛋白（原）降解产物的鉴定与分布。单克隆抗体的应用。
Arteriosclerosis. 1989 Jan-Feb;9(1):109-21. doi: 10.1161/01.atv.9.1.109.
4
New strategies in the determination of fibrin and fibrin(ogen) derivatives by monoclonal antibodies.利用单克隆抗体测定纤维蛋白和纤维蛋白（原）衍生物的新策略。
Blut. 1988 Nov;57(5):285-91. doi: 10.1007/BF00320354.
5
A sensitive latex slide assay for fibrin(ogen) fragment E.
Clin Chim Acta. 1985 Apr 15;147(2):173-7. doi: 10.1016/0009-8981(85)90079-8.
6
No influence of heparin plasma and other (pre)analytic variables on D-dimer determinations.
Clin Chem. 2002 Sep;48(9):1611-3.
7
Blood coagulation after long distance running: antithrombin III prevents fibrin formation.长跑后的血液凝固：抗凝血酶III可防止纤维蛋白形成。
Thromb Haemost. 1990 Jun 28;63(3):430-4.
8
Pre-analytical variation in the measurements of sensitive markers of coagulation and fibrinolysis: the influence of venipuncture and mixing of blood.
Haemostasis. 1997 May-Jun;27(3):119-24. doi: 10.1159/000217443.
9
Characterisation of serum fibrinogen degradation products using gel chromatography and radioimmunoassay.
Thromb Haemost. 1977 Jun 30;37(3):471-6.
10
Sequence of fibrinogen proteolysis and platelet release after intrauterine infusion of hypertonic saline.宫内输注高渗盐水后纤维蛋白原蛋白水解及血小板释放的顺序
J Clin Invest. 1979 Nov;64(5):1371-8. doi: 10.1172/JCI109594.
本文引用的文献
1
DEMONSTRATION OF FIBRINOLYTIC SPLIT PRODUCTS IN HUMAN SERUM BY AN IMMUNOLOGICAL METHOD IN SPONTANEOUS AND INDUCED FIBRINOLYTIC STATES.用免疫方法在自发和诱导纤溶状态下检测人血清中的纤维蛋白溶解分裂产物
Scand J Haematol. 1964;1:313-30. doi: 10.1111/j.1600-0609.1964.tb00029.x.
2
[Free fibrin in the circulation. Immunological test for the identification of free fibrin].
Rev Paul Med. 1961 Aug;59:103-5.
3
4
Split products of fibrin in maternal serum in the perinatal period.
Am J Obstet Gynecol. 1970 Nov 15;108(6):941-5. doi: 10.1016/0002-9378(70)90338-8.
5
Fibrin degradation products in pre-eclamptic toxaemia and eclampsia.先兆子痫和子痫中的纤维蛋白降解产物
Br Med J. 1970 Sep 5;3(5722):545-7. doi: 10.1136/bmj.3.5722.545.
6
Fibrin degradation products in normal and abnormal pregnancy and parturition.正常与异常妊娠及分娩中的纤维蛋白降解产物
Br Med J. 1969 Jul 19;3(5663):137-40. doi: 10.1136/bmj.3.5663.137.
7
Detection of fibrin(ogen) degradation products by a latex clumping method.
Scand J Haematol Suppl. 1971;13:115-9. doi: 10.1111/j.1600-0609.1971.tb01996.x.
8
Serum and urine fibrinogen-fibrin-related antigen (F.R.-antigen) levels in renal disease.肾脏疾病中血清及尿液纤维蛋白原-纤维蛋白相关抗原（F.R.-抗原）水平
Br Med J. 1972 Oct 14;4(5832):82-5. doi: 10.1136/bmj.4.5832.82.
9
Increase in fibrinogen and fibrin-related antigen in human serum due to in vitro lysis of fibrin by thrombin.凝血酶体外溶解纤维蛋白导致人血清中纤维蛋白原和纤维蛋白相关抗原增加。
J Clin Invest. 1972 Apr;51(4):903-11. doi: 10.1172/JCI106885.
10
Intravascular coagulation during pregnancy and the puerperium.妊娠及产褥期的血管内凝血
J Obstet Gynaecol Br Commonw. 1973 Dec;80(12):1059-66. doi: 10.1111/j.1471-0528.1973.tb02980.x.
Zotero 插件