Asanza Teruel M L, Gontier E, Bienaime C, Nava Saucedo J E, Barbotin J N
Laboratoire de Génie Cellulaire, UPRES A CNRS 6022, Faculté des Sciences, Université de Picardie Jules Verne, Amiens, France.
Enzyme Microb Technol. 1997 Oct;21(5):314-20. doi: 10.1016/s0141-0229(97)00045-8.
A full-factorial experimental design at three levels with two independent variables, carrageenan concentration (1.0, 1.5, and 2.0%) and potassium chloride concentration (0.3, 0.7, and 1.1 M) was studied in order to analyze the effect of both factors on the antibiotic production of K-carrageenan-immobilized mycelia of Streptomyces aureofaciens. The response surfaces obtained have indicated that both carrageenan and potassium chloride concentrations have a pronounced effect on the yield of chlortetracycline (CTC) and tetracycline (TC) produced by S. aureofaciens. By exclusively varying the immobilization conditions, the tetracycline production can be enhanced more than eight times (12.3 mg g-1 biomass for immobilized cells vs. 1.5 mg g-1 biomass for free cells) in comparison with free-cell mycelial cultures.
为了分析角叉菜胶浓度（1.0%、1.5%和2.0%）和氯化钾浓度（0.3M、0.7M和1.1M）这两个自变量对金色链霉菌K-角叉菜胶固定化菌丝体抗生素产量的影响，研究了一种具有三个水平的全因子实验设计。所得响应面表明，角叉菜胶和氯化钾浓度对金色链霉菌产生的金霉素（CTC）和四环素（TC）产量均有显著影响。与游离细胞菌丝体培养相比，仅通过改变固定化条件，四环素产量可提高八倍以上（固定化细胞为12.3mg g-1生物量，游离细胞为1.5mg g-1生物量）。
