Al-Ibrahim M S, Chandra R, Kishore R, Valentine F T, Lawrence H S
J Immunol Methods. 1976 Mar;10(2-3):207-18. doi: 10.1016/0022-1759(76)90172-1.
Studies in vitro of human macrophage function in health and disease have been impeded by the difficulty of obtaining such cells in sufficient number. Unlike animal species, the only readily available source of human macrophages are circulating monocytes. Herein, a method is described whereby the phagocytic rate of small numbers of glass-adherent mononuclear cells can be accurately measured. The method utilizes the ingestion by macrophages of technetium labelled polystyrene particles; both the radiolabel and ingestible substrate are readily available and the labelling process simple and efficient. The phagocytic rate can be expressed as radioactive counts per microgram of cell protein; data is also presented showing that the number of particles ingested per cell can be accurately derived.
在健康和疾病状态下对人类巨噬细胞功能进行体外研究一直受到难以获得足够数量此类细胞的阻碍。与动物物种不同，人类巨噬细胞唯一容易获得的来源是循环单核细胞。本文描述了一种方法，通过该方法可以准确测量少量玻璃贴壁单核细胞的吞噬率。该方法利用巨噬细胞摄取锝标记的聚苯乙烯颗粒；放射性标记物和可摄取底物都很容易获得，并且标记过程简单有效。吞噬率可以表示为每微克细胞蛋白的放射性计数；还给出了数据表明每个细胞摄取的颗粒数量可以准确得出。
