Lehnert B E, Tech C
J Immunol Methods. 1985 Apr 22;78(2):337-44. doi: 10.1016/0022-1759(85)90090-0.
Macrophages can bind and engulf a variety of particles in the absence of specific opsonins. Polystyrene-type microspheres are often employed to quantitate opsonin-independent phagocytic activities of macrophages in vitro. Reliable measurement of this cell function, however, requires the ability of the investigator to distinguish between particles that are merely attached to the cell surface and those that are actually internalized. We have developed a simple, rapid, and reproducible method for quantitating phagocytosis using polystyrene microspheres and adherent alveolar macrophages. Basically, particles associated with macrophages after a given incubation time are microscopically quantitated on a cell-by-cell basis before and after toluene dissolution of external particles. Particle/macrophage values obtained after toluene treatment exclusively index phagocytosis.
巨噬细胞在没有特异性调理素的情况下可以结合并吞噬多种颗粒。聚苯乙烯型微球常被用于体外定量巨噬细胞不依赖调理素的吞噬活性。然而，要可靠地测量这种细胞功能，研究人员需要有能力区分仅仅附着在细胞表面的颗粒和实际内化的颗粒。我们开发了一种简单、快速且可重复的方法，使用聚苯乙烯微球和贴壁肺泡巨噬细胞来定量吞噬作用。基本上，在给定的孵育时间后与巨噬细胞相关的颗粒，在外部颗粒经甲苯溶解前后，逐个细胞地进行显微镜定量。甲苯处理后获得的颗粒/巨噬细胞值专门用于指示吞噬作用。
