Popov V N, Simonian R A, Skulachev V P, Starkov A A
Biological Department, Voronezh State University, Russia.
FEBS Lett. 1997 Sep 22;415(1):87-90. doi: 10.1016/s0014-5793(97)01099-5.
The hypothesis that a non-coupled alternative oxidase of plant mitochondria operates as an antioxygen defence mechanism [Purvis, A.C. and Shewfelt, R.L., Physiol. Plant. 88 (1993) 712-718; Skulachev, V.P., Biochemistry (Moscow) 59 (1994) 1433-1434] has been confirmed in experiments on isolated soybean and pea cotyledon mitochondria. It is shown that inhibitors of the alternative oxidase, salicyl hydroxamate and propyl gallate strongly stimulate H2O2 production by these mitochondria oxidizing succinate. Effective concentrations of the inhibitors proved to be the same as those decreasing the cyanide-resistant respiration. The inhibitors proved to be ineffective in stimulating H2O2 formation in rat liver mitochondria lacking the alternative oxidase.
植物线粒体的非偶联交替氧化酶作为一种抗氧防御机制的假说[Purvis, A.C.和Shewfelt, R.L., Physiol. Plant. 88 (1993) 712 - 718; Skulachev, V.P., Biochemistry (Moscow) 59 (1994) 1433 - 1434]已在对分离的大豆和豌豆子叶线粒体进行的实验中得到证实。结果表明，交替氧化酶的抑制剂水杨基羟肟酸和没食子酸丙酯能强烈刺激这些氧化琥珀酸的线粒体产生H2O2。抑制剂的有效浓度与降低抗氰呼吸的浓度相同。事实证明，这些抑制剂对缺乏交替氧化酶的大鼠肝线粒体中H2O2的形成没有刺激作用。
