Popov V N, Ruuge E K, Starkov A A
Department of Plant Physiology and Biochemistry, Voronezh State University, Voronezh, 394693, Russia.
Biochemistry (Mosc). 2003 Jul;68(7):747-51. doi: 10.1023/a:1025078815819.
The effect of inhibitors of the cytochrome pathway and alternative oxidase on the rate of respiration and generation of reactive oxygen species by pea mitochondria was studied. Respiration of mitochondria from pea cotyledons was inhibited by 70-80% by salicylhydroxamate (SHAM). The rate of hydrogen peroxide production by pea cotyledon mitochondria during succinate oxidation was 0.15 nmol/min per mg protein. SHAM considerably accelerated the hydrogen peroxide production. The SHAM-dependent H2O2 production was stimulated by 2 micro M antimycin A and inhibited by 5 mM KCN and 1 micro M myxothiazol. The study of the rate of O2*- generation by pea mitochondria using EPR spin traps and epinephrine oxidation showed that H2O2 accumulation can be accounted for by a significant increase in the rate of O2*- production.
研究了细胞色素途径抑制剂和交替氧化酶对豌豆线粒体呼吸速率和活性氧生成的影响。水杨基羟肟酸（SHAM）可使豌豆子叶线粒体的呼吸受到70 - 80%的抑制。豌豆子叶线粒体在琥珀酸氧化过程中过氧化氢的产生速率为每毫克蛋白质0.15纳摩尔/分钟。SHAM显著加速了过氧化氢的产生。2微摩尔的抗霉素A刺激了依赖SHAM的H2O2产生，而5毫摩尔的KCN和1微摩尔的粘噻唑则抑制了该产生。使用电子顺磁共振自旋捕集技术和肾上腺素氧化对豌豆线粒体O2*-生成速率的研究表明，H2O2的积累可归因于O2*-产生速率的显著增加。
