Partidos C D, Vohra P, Jones D, Farrar G, Steward M W
Department of Pathology and Infectious Diseases, Royal Veterinary College, London, UK.
J Immunol Methods. 1997 Aug 7;206(1-2):143-51. doi: 10.1016/s0022-1759(97)00102-6.
A synthetic peptide representing a measles virus (MV) cytotoxic T cell epitope (CTL) when encapsulated in poly (D,L-lactide co-glycolide) (PLG) 50:50 microparticles induced a strong CTL response after a single intraperitoneal immunization of mice which was greater than that following administration of the peptide in Freund's complete adjuvant. A 100 micrograms dose of encapsulated peptide was shown to be more effective for CTL priming than 50 and 25 micrograms doses. A vaccine formulation prepared by simply mixing empty 50:50 PLG microparticles with the peptide resulted in the induction of CTL responses comparable to those induced by the encapsulated peptide. Moreover, a CTL response against MV-infected target cells was observed. These findings highlight the potential immunostimulatory effect of PLG microparticles for the induction of MV and peptide-specific CTL responses.
当包裹在聚（D，L-丙交酯乙交酯）（PLG）50:50微颗粒中的代表麻疹病毒（MV）细胞毒性T细胞表位（CTL）的合成肽经腹腔单次免疫小鼠后，可诱导强烈的CTL反应，该反应强于在弗氏完全佐剂中给予该肽后的反应。已表明100微克剂量的包裹肽对CTL启动比50微克和25微克剂量更有效。通过简单地将空的50:50 PLG微颗粒与肽混合制备的疫苗制剂可诱导与包裹肽诱导的CTL反应相当的反应。此外，观察到针对MV感染靶细胞的CTL反应。这些发现突出了PLG微颗粒对诱导MV和肽特异性CTL反应的潜在免疫刺激作用。
