Weddle O H, Mason W D
J Pharm Sci. 1976 Jun;65(6):865-8. doi: 10.1002/jps.2600650616.
A rapid, specific, high-pressure liquid chromatographic method for the determination of theophylline in plasma was developed. The procedure is fast enough (21 min from receipt of blood to reporting value) to be used for emergency determinations. The sensitivity, precision, and accuracy are sufficient for routine monitoring of therapeutic levels in patients. The assay is specific enough to be valid in the presence of caffeine and theobromine. Metabolites of theophylline as well as a number of drugs do not interfere with the assay.
建立了一种快速、特异的高压液相色谱法测定血浆中的茶碱。该方法速度足够快（从收到血样到报告结果只需21分钟），可用于紧急测定。其灵敏度、精密度和准确度足以用于常规监测患者的治疗水平。该测定法特异性高，在咖啡因和可可碱存在的情况下仍有效。茶碱的代谢产物以及多种药物均不干扰该测定。
