De Souza W, Bunn M M, Angluster J
J Protozool. 1976 May;23(2):329-33. doi: 10.1111/j.1550-7408.1976.tb03781.x.
Leptomonas pessoai promastigotes cultivated in a synthetic medium were agglutinated with concanavalin A (ConA). Agglutination was predominantly of the flagellar-flagellar type, and was inhibited with sucrose or alpha-methyl-D-mannoside. Cell surface polysaccharides and/or glycoproteins were demonstrated by several cytochemical methods at the fine-structural level. A Con A-horseradish peroxidase-diaminobenzidine (DAB) technic was used to detect Con A receptors in the pellicular membrane of the flagellar pocket region and in the flagellar membrane proper. Somewhat less of the Con A-DAB reaction product was observed in the pellicular membrane enclosing the rest of the cell.
在合成培养基中培养的佩索利细滴虫前鞭毛体与伴刀豆球蛋白A（ConA）发生凝集。凝集主要为鞭毛-鞭毛型，可被蔗糖或α-甲基-D-甘露糖苷抑制。通过几种细胞化学方法在精细结构水平上证实了细胞表面多糖和/或糖蛋白的存在。采用Con A-辣根过氧化物酶-二氨基联苯胺（DAB）技术检测鞭毛袋区域的表膜和鞭毛膜本身中的Con A受体。在包裹细胞其余部分的表膜中观察到的Con A-DAB反应产物略少。
