Schottelius J
Z Parasitenkd. 1982;66(3):237-47. doi: 10.1007/BF00925341.
Four-day-old promastigote culture forms of L. tropica major from USSR (LV-252, LV-253, LRC L-38) and Saudi Arabia (Kö, Ha), L. tropica minor from USSR (LV-239, LRC L-39) Leishmania sp. from Israel (Ro) and Saudi Arabia (Ve-322, Schwe, Ne), L. donovani from Sudan (3S, 1-S, LV-139) and India (LV-125, LRC L-51), L donovani infantum from Israel (LV-140), and L. aethiopica from Ethiopia (LV-1, LV-16, LV-24, LV-26) were tested using the following lectins: C. ensiformis, R. communis-120, A. polypoides, P. vulgaris, E. europaeus, D. biflorus, L. tetragonolobus, U. europaeus, L. alpinum, A. papillata II, A. hypogaea, and S. hispida. All strains reacted with C. ensiformis, R. communis-120, and A. polypoides. No agglutination reactions were observed with P. vulgaris, D. biflorus, E. europaeus, and L. tetragonolobus. Agglutination differences were detected by reactions with A. papillata II, U. europaeus, L. alpinum, A. hypogaea, and S. hispida. L. tropica minor (LRC L-39, LV-249), L. donovani (LV-239, 1-S, 3S, LRC L-51), L. aethiopica (LV-1, LV-15, LV-24, LV-26), and L. tropica major (LB-242, LV-253, LRC LK-38, Kö, Ha) are distinguishable with lectins. From L. tropica major two intraspecific forms can be identified: USSR-type (LV-252, LV-253, LRC L-38) and a Near East-type (Kö, Ha). The Leishmania sp. strains (Ve-322, Ne, Ro, Schwe) belong to the Near East-type. The strains L. donovano LV-140 and L. donovani LV-125 react as L. tropica minor, a fact which cannot be elucidated. The L. donovani strains from Sudan cannot be distinguished from the Indian strain L. donovani LRC L-51.
使用以下凝集素对来自苏联（LV - 252、LV - 253、LRC L - 38）和沙特阿拉伯（Kö、Ha）的热带利什曼原虫大型前鞭毛体培养物、来自苏联（LV - 239、LRC L - 39）的热带利什曼原虫小型前鞭毛体、来自以色列（Ro）和沙特阿拉伯（Ve - 322、Schwe、Ne）的利什曼原虫属、来自苏丹（3S、1 - S、LV - 139）和印度（LV - 125、LRC L - 51）的杜氏利什曼原虫、来自以色列（LV - 140）的婴儿利什曼原虫以及来自埃塞俄比亚（LV - 1、LV - 16、LV - 24、LV - 26）的埃塞俄比亚利什曼原虫进行了测试：剑豆凝集素、蓖麻凝集素 - 120、多花菜豆凝集素、普通菜豆凝集素、欧洲七叶树凝集素、双花扁豆凝集素、四棱豆凝集素、欧洲榛凝集素、高山豆凝集素、乳头紫云英凝集素II、花生凝集素和硬毛茄凝集素。所有菌株均与剑豆凝集素、蓖麻凝集素 - 120和多花菜豆凝集素发生反应。普通菜豆凝集素、双花扁豆凝集素、欧洲七叶树凝集素和四棱豆凝集素未观察到凝集反应。通过与乳头紫云英凝集素II、欧洲榛凝集素、高山豆凝集素、花生凝集素和硬毛茄凝集素的反应检测到凝集差异。热带利什曼原虫小型（LRC L - 39、LV - 249）、杜氏利什曼原虫（LV - 239、1 - S、3S、LRC L - 51）、埃塞俄比亚利什曼原虫（LV - 1、LV - 15、LV - 24、LV - 26）和热带利什曼原虫大型（LB - 242、LV - 253、LRC LK - 38、Kö、Ha）可用凝集素区分。从热带利什曼原虫大型中可鉴定出两种种内形式：苏联型（LV - 252、LV - 253、LRC L - 38）和近东型（Kö、Ha）。利什曼原虫属菌株（Ve - 322、Ne、Ro、Schwe）属于近东型。杜氏利什曼原虫LV - 140菌株和杜氏利什曼原虫LV - 125菌株的反应与热带利什曼原虫小型相同，这一事实尚无法解释。来自苏丹的杜氏利什曼原虫菌株与印度的杜氏利什曼原虫LRC L - 51菌株无法区分。
