Koppel A M, Feiner L, Kobayashi H, Raper J A
Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Neuron. 1997 Sep;19(3):531-7. doi: 10.1016/s0896-6273(00)80369-4.
The semaphorin family contains secreted and transmembrane signaling proteins that function in the nervous, immune, and cardiovascular systems. Chick collapsin-1 is a repellent for specific growth cones. Two other secreted members of the semaphorin family, collapsin-2 and -3, are structurally similar to collapsin-1 but have different biological activities. Semaphorins contain a 500 amino acid family signature semaphorin domain. We show in this study that (1) the semaphorin domain of collapsin-1 is both necessary and sufficient for biological activity, (2) the semaphorin domain contains a 70 amino acid region that specifies the biological activity of the three family members, and (3) the positively charged carboxy terminus potentiates activity without affecting specificity. We propose that semaphorins interact with their receptors through two independent binding sites: one that mediates the biological response and one that potentiates it.
信号素家族包含在神经、免疫和心血管系统中发挥作用的分泌型和跨膜信号蛋白。鸡源塌陷素-1是特定生长锥的排斥因子。信号素家族的另外两个分泌型成员，塌陷素-2和-3，在结构上与塌陷素-1相似，但具有不同的生物学活性。信号素含有一个由500个氨基酸组成的家族标志性信号素结构域。我们在本研究中表明：（1）塌陷素-1的信号素结构域对生物学活性既是必需的也是充分的；（2）信号素结构域包含一个由70个氨基酸组成的区域，该区域决定了这三个家族成员的生物学活性；（3）带正电荷的羧基末端增强活性而不影响特异性。我们提出，信号素通过两个独立的结合位点与其受体相互作用：一个介导生物学反应，另一个增强该反应。
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