Rosenblum L L, Supriatna J, Melnick D J
Department of Anthropology, Columbia University, New York, New York 10027, USA.
Am J Phys Anthropol. 1997 Sep;104(1):35-45. doi: 10.1002/(SICI)1096-8644(199709)104:1<35::AID-AJPA3>3.0.CO;2-C.
Mitochondrial DNA variation was surveyed in nine populations of the pigtail macaque (Macaca nemestrina), covering all three recognized subspecies in Southeast Asia. To do this, a 2,300 base pair fragment spanning the mitochondrial NAD 3 and NAD 4 genes and flanking tRNA subunits leucine and glycine was targeted for amplification and digested with a battery of 16 restriction endonucleases. Out of a total of 107 individuals, 32 unique haplotypes could be distinguished. Parsimony and neighbor-joining analyses grouped the haplotypes into five strongly supported assemblages representing China/Thailand, Malaysia, Sumatra, Borneo, and Siberut. These results indicate that the mainland and island mtDNA haplotypes are strictly and uniquely limited to the geographic ranges of the recognized morphological subspecies. Cladistic and neighbor-joining analyses indicate that inferred phylogenies of mtDNA haplotypes are congruent with subspecies designations. Furthermore, in support of morphological studies, results indicate that the Mentawai macaque is most likely not a distinct species but a subspecies of M. nemestrina.
对猪尾猕猴（Macaca nemestrina）的九个种群的线粒体DNA变异情况进行了调查，这些种群涵盖了东南亚所有三个公认的亚种。为此，靶向扩增了一段跨越线粒体NAD 3和NAD 4基因以及侧翼亮氨酸和甘氨酸tRNA亚基的2300个碱基对的片段，并用一组16种限制性内切酶进行消化。在总共107个个体中，可以区分出32种独特的单倍型。简约分析和邻接法分析将这些单倍型分为五个得到有力支持的组合，分别代表中国/泰国、马来西亚、苏门答腊、婆罗洲和西比路岛。这些结果表明，大陆和岛屿的线粒体DNA单倍型严格且独特地局限于公认形态亚种的地理范围内。分支分析和邻接法分析表明，推断的线粒体DNA单倍型系统发育与亚种分类一致。此外，为支持形态学研究，结果表明明打威猕猴很可能不是一个独特的物种，而是猪尾猕猴的一个亚种。
