Fischer J A, Leavell S K, Li Q
Department of Zoology, University of Texas at Austin 78712, USA.
Dev Genet. 1997;21(2):167-74. doi: 10.1002/(SICI)1520-6408(1997)21:2<167::AID-DVG6>3.0.CO;2-5.
The Drosophila fat facets gene encodes a deubiquitinating enzyme that regulates a cell communication pathway essential early during eye development to inhibit the determination of excess photoreceptors. Ubiquitin is a small polypeptide that tags proteins for degradation by a multisubunit proteolytic complex called the proteasome. The FAT FACETS protein is thought to be required to remove ubiquitin from a particular protein, thereby rescuing if from proteolysis. In order to identify the genes encoding the substrate of FAT FACETS and other components of the neural inhibition pathway, a mutagenesis screen for dominant enhancers of the fat facets mutant eye phenotype was performed. Several genes were identified, one of which is an excellent candidate for encoding a component of the pathway regulated by FAT FACETS. Three different eye phenotypes were observed when the fat facets mutants were dominantly enhanced by different mutations, suggesting that fat facets has other functions in addition to its critical role early in eye development.
果蝇的胖小眼基因编码一种去泛素化酶，该酶在眼睛发育早期调节一条细胞通讯途径，以抑制过多光感受器的确定。泛素是一种小多肽，它标记蛋白质以便被称为蛋白酶体的多亚基蛋白水解复合物降解。人们认为胖小眼蛋白需要从特定蛋白质上去除泛素，从而使其免遭蛋白水解。为了鉴定编码胖小眼底物和神经抑制途径其他成分的基因，对胖小眼突变体眼睛表型的显性增强子进行了诱变筛选。鉴定出了几个基因，其中一个是编码由胖小眼调节的途径成分的极佳候选基因。当胖小眼突变体被不同突变显性增强时，观察到了三种不同的眼睛表型，这表明胖小眼除了在眼睛发育早期起关键作用外，还有其他功能。
