Zhang S, Cordon-Cardo C, Zhang H S, Reuter V E, Adluri S, Hamilton W B, Lloyd K O, Livingston P O
Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Int J Cancer. 1997 Sep 26;73(1):42-9. doi: 10.1002/(sici)1097-0215(19970926)73:1<42::aid-ijc8>3.0.co;2-1.
Understanding the distribution of tumor-associated antigens on cancers and normal tissues is essential for selection of targets for cancer immunotherapy. Seven carbohydrate antigens, potential targets for immunotherapy, were studied using a panel of well-characterized MAbs by immunohistochemistry on cryostat-cut tissue sections of 13 types of cancers and 18 normal tissues. GD2 and GD3 were present on most cancers of neuroectodermal origin and GD2 was also present on B cell lymphomas. 9-O-acetyl-GD3 was detected only on melanoma while fucosyl GM1 was detected only on small cell lung cancers (SCLC). Surprisingly, GM2 was strongly expressed on all tested tumors, including cancers of neuroectodermal origin and cancers of epithelial origin. Polysialic acid was primarily expressed on SCLC and neuroblastomas. Globo H was present on most cancers of epithelial origin. These antigens were also identified in normal tissues. Fucosyl GM1 was not expressed significantly on any of the normal tissues analyzed. GD3, GD2, GM2 and polysialic acid were detected in normal brain to varying degrees. GM2 and Globo H were expressed on the luminal surface of epithelia of a variety of organs. The unexpected expression of GM2 on a broad range of cancers and normal epithelial tissues was confirmed by loss after methanol fixation and by immune thin layer chromatography.
了解肿瘤相关抗原在癌症组织和正常组织中的分布情况对于癌症免疫治疗靶点的选择至关重要。使用一组经过充分表征的单克隆抗体，通过免疫组织化学方法，在13种癌症和18种正常组织的低温切片上研究了7种碳水化合物抗原（免疫治疗的潜在靶点）。GD2和GD3存在于大多数神经外胚层起源的癌症中，GD2也存在于B细胞淋巴瘤中。9-O-乙酰-GD3仅在黑色素瘤中检测到，而岩藻糖基GM1仅在小细胞肺癌（SCLC）中检测到。令人惊讶的是，GM2在所有测试的肿瘤中均强烈表达，包括神经外胚层起源的癌症和上皮起源的癌症。聚唾液酸主要表达于小细胞肺癌和神经母细胞瘤中。Globo H存在于大多数上皮起源的癌症中。这些抗原在正常组织中也有发现。在所分析的任何正常组织中，岩藻糖基GM1均未显著表达。GD3、GD2、GM2和聚唾液酸在正常脑组织中均有不同程度的检测到。GM2和Globo H在多种器官上皮的腔表面表达。甲醇固定后GM2的消失以及免疫薄层色谱法证实了GM2在广泛的癌症和正常上皮组织中的意外表达。
