Raengsakulrach B, Ong-aj-yooth L, Thaiprasert T, Nilwarangkur S, Ong-aj-yooth S, Narupiti S, Thirawuth V, Klungthong C, Snitbhan R, Vaughn D W
Department of Virology, U.S. Army Medical Component, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
J Med Virol. 1997 Oct;53(2):162-6. doi: 10.1002/(sici)1096-9071(199710)53:2<162::aid-jmv9>3.0.co;2-7.
Patients receiving kidney transplants (KT) are at high risk for blood borne viral infections. To determine the prevalence of a recently discovered hepatitis G virus (HGV) in this patient group, reverse transcription-polymerase chain reaction (RT-PCR) employing primers derived from the NS5 region of the viral genome was utilized. HGV RNA was detected in 40 of 94 KT patients (43%), as compared to 3 of 69 healthy subjects (4.3%). Cocirculation of HGV and hepatitis C virus (HCV) RNA was detected in 12 patients (13%). Comparison of patients with and without HGV revealed that the former had received hemodialysis before transplantation for a significantly longer duration than the latter (28 vs. 17 months, respectively; P < 0.05). The amount of blood transfused and mean levels of liver enzymes, including alkaline phosphatase, alanine transaminase, and aspartate transaminase, were the same in both groups. Sequence analysis of 275-base pair DNA clones obtained from 2 patients revealed approximately 92% sequence homology to the published HGV and GB virus C sequences. These results suggested that HGV infection among Thai KT patients was high and the role of HGV in causing liver disease remains to be determined.
接受肾移植（KT）的患者面临血源性病毒感染的高风险。为了确定该患者群体中最近发现的庚型肝炎病毒（HGV）的流行情况，采用了源自病毒基因组NS5区域的引物进行逆转录聚合酶链反应（RT-PCR）。在94名KT患者中有40名（43%）检测到HGV RNA，相比之下，69名健康受试者中有3名（4.3%）检测到。在12名患者（13%）中检测到HGV和丙型肝炎病毒（HCV）RNA的共同循环。对有和没有HGV的患者进行比较发现，前者在移植前接受血液透析的时间明显长于后者（分别为28个月和17个月；P<0.05）。两组患者的输血量以及包括碱性磷酸酶、丙氨酸转氨酶和天冬氨酸转氨酶在内的肝酶平均水平相同。对从2名患者获得的275个碱基对DNA克隆进行的序列分析显示，与已发表的HGV和GB病毒C序列的序列同源性约为92%。这些结果表明，泰国KT患者中HGV感染率很高，HGV在引起肝病中的作用尚待确定。
