Black G W, Rixon J E, Clarke J H, Hazlewood G P, Ferreira L M, Bolam D N, Gilbert H J
Department of Biological and Nutritional Sciences, University of Newcastle upon Tyne, UK.
J Biotechnol. 1997 Sep 16;57(1-3):59-69. doi: 10.1016/s0168-1656(97)00089-8.
To evaluate the role of the CBDs and linker sequences in Pseudomonas xylanase A (XYLA) and arabinofuranosidase C (XYLC), the catalytic activity of derivatives of these enzymes, lacking either the linker sequences or CBDs, was assessed. Removal of the CBDs or linker sequences did not affect the activity of either XYLA or XYLC against soluble arabinoxylan, while derivatives of XYLA, in which either the CBD or interdomain regions had been deleted, exhibited decreased activity against the xylan component of cellulose/hemicellulose complexes. Although a truncated derivative of XYLC (XYLC"'), lacking its CBD, was less active than the full-length enzyme against plant cell wall material containing highly substituted arabinoxylan, XYLC"' was more active than XYLC on complex substrates where the degree of substitution of arabinoxylan was very low. These data indicate that CBDs and linker sequences play an important role in the activity of hemicellulases against plant cell walls and other cellulose/hemicellulose complexes.
为了评估纤维素结合结构域（CBDs）和连接序列在木糖酶A（XYLA）和阿拉伯呋喃糖苷酶C（XYLC）中的作用，对这些酶缺失连接序列或CBDs的衍生物的催化活性进行了评估。去除CBDs或连接序列并不影响XYLA或XYLC对可溶性阿拉伯木聚糖的活性，而XYLA的衍生物，其中CBD或结构域间区域被删除，对纤维素/半纤维素复合物的木聚糖成分的活性降低。虽然缺失其CBD的XYLC截短衍生物（XYLC'''）对含有高度取代阿拉伯木聚糖的植物细胞壁材料的活性低于全长酶，但XYLC'''在阿拉伯木聚糖取代度非常低的复合底物上比XYLC更具活性。这些数据表明，CBDs和连接序列在半纤维素酶对植物细胞壁和其他纤维素/半纤维素复合物的活性中起重要作用。
