Rhee J K, Kim H C, Park B K
Bio-Safety Research Institute, Chonbuk National University, Chonju, Korea.
Korean J Parasitol. 1997 Sep;35(3):181-7. doi: 10.3347/kjp.1997.35.3.181.
In order to clarify the effect of cryptosporidiosis on immune response, histopathological changes associated with experimentally occurring bursal cryptosporidiosis in chickens were chronologically observed as the first step. A total of 150 2-day-old chickens was each inoculated orally with a single dose of 5 x 10(5) Cryptosporidium baileyi oocysts. The chickens showed a normal profile of oocyst shedding in droppings. The bursa indices throughout the experimental period indicated negligible reactions. Numerous cryptosporidia occurred in the microvillous border of bursal epithelium between days 4 and 16 postinoculation (PI). Appearance of the most mast cells was followed by a dramatic loss of the protozoa in the bursa of Fabricius (BF). The distribution of the coccidium coincided with heterophil infiltration in the epithelium and adjacent lamina propria. The histopathological lesion was marked diffuse chronic superficial purulent bursitis with heterophil infiltration in the epithelium and adjacent lamina propria and mucosal epithelial hyperplasia. These results suggest that the bursitis may induce immunosuppressive effect.
为了阐明隐孢子虫病对免疫反应的影响，第一步按时间顺序观察了与实验性鸡法氏囊隐孢子虫病相关的组织病理学变化。总共150只2日龄鸡，每只口服接种单剂量5×10⁵个贝氏隐孢子虫卵囊。鸡粪便中的卵囊排出情况正常。整个实验期间法氏囊指数显示反应可忽略不计。接种后第4天至第16天，法氏囊上皮微绒毛边界出现大量隐孢子虫。大多数肥大细胞出现后，法氏囊中原生动物数量急剧减少。球虫的分布与上皮和相邻固有层中的嗜异性粒细胞浸润一致。组织病理学病变为明显的弥漫性慢性浅表化脓性法氏囊炎，上皮和相邻固有层有嗜异性粒细胞浸润以及黏膜上皮增生。这些结果表明，法氏囊炎可能诱导免疫抑制作用。
