Ghazizadeh M, Miyata N, Sasaki Y, Arai K, Aihara K
Central Institute for Electron Microscopic Researches, Nippon Medical School, Tokyo, Japan.
Am J Dermatopathol. 1997 Oct;19(5):468-72. doi: 10.1097/00000372-199710000-00006.
Silver-stained nucleolar organizer regions (AgNORs) have been widely used as a marker of cellular activity and proliferation. In a retrospective study, we investigated the potential value of AgNORs in 12 hypertrophic and 24 keloid scar tissues. Ten normal skin tissues served as controls. A standard silver-staining method was used, and the mean AgNOR count of dermal fibroblastic cells in each tissue was determined. In normal skin, the mean AgNOR count of dermal fibroblasts was 1.79+/-0.55, whereas fibroblastic cells in hypertrophic and keloid scars had mean AgNOR counts of 3.18+/-0.56 and 5.10+/-0.97, respectively. There was a statistically significant difference between the mean AgNOR counts of fibroblastic cells from normal skin, hypertrophic scar, and keloid scar [one-factor analysis of variance (ANOVA), p < 0.0001]. Our findings suggest that AgNOR count may be a useful marker for assessment of fibroblastic cell activity in hypertrophic and keloid scars, which may have potential value for histologic and biologic characterization of the two lesions.
银染核仁组成区（AgNORs）已被广泛用作细胞活性和增殖的标志物。在一项回顾性研究中，我们调查了AgNORs在12个肥厚性瘢痕组织和24个瘢痕疙瘩组织中的潜在价值。选取10个正常皮肤组织作为对照。采用标准银染方法，测定每个组织中真皮成纤维细胞的平均AgNOR计数。在正常皮肤中，真皮成纤维细胞的平均AgNOR计数为1.79±0.55，而肥厚性瘢痕和瘢痕疙瘩中的成纤维细胞平均AgNOR计数分别为3.18±0.56和5.10±0.97。正常皮肤、肥厚性瘢痕和瘢痕疙瘩中成纤维细胞的平均AgNOR计数之间存在统计学显著差异[单因素方差分析（ANOVA），p<0.0001]。我们的研究结果表明，AgNOR计数可能是评估肥厚性瘢痕和瘢痕疙瘩中成纤维细胞活性的有用标志物，这可能对这两种病变的组织学和生物学特征具有潜在价值。
