• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Mechanism of decay of the cry1Aa mRNA in Bacillus subtilis.枯草芽孢杆菌中cry1Aa mRNA的衰变机制。
J Bacteriol. 1997 Oct;179(20):6341-8. doi: 10.1128/jb.179.20.6341-6348.1997.
2
cry1Aa lacks stability elements at its 5'-UTR but integrity of its transcription terminator is critical to prevent decay of its transcript.cry1Aa在其5'-非翻译区缺乏稳定性元件，但其转录终止子的完整性对于防止其转录本降解至关重要。
Curr Microbiol. 2006 Jul;53(1):23-9. doi: 10.1007/s00284-005-5178-1. Epub 2006 Jun 6.
3
Vegetative expression of the delta-endotoxin genes of Bacillus thuringiensis subsp. kurstaki in Bacillus subtilis.苏云金芽孢杆菌库尔斯塔克亚种δ-内毒素基因在枯草芽孢杆菌中的营养期表达
J Bacteriol. 1986 Apr;166(1):194-204. doi: 10.1128/jb.166.1.194-204.1986.
4
Expression in Bacillus subtilis of the Bacillus thuringiensis cryIIIA toxin gene is not dependent on a sporulation-specific sigma factor and is increased in a spo0A mutant.苏云金芽孢杆菌cryIIIA毒素基因在枯草芽孢杆菌中的表达不依赖于芽孢形成特异性σ因子，且在spo0A突变体中表达增加。
J Bacteriol. 1994 Aug;176(15):4734-41. doi: 10.1128/jb.176.15.4734-4741.1994.
5
In vitro analysis of mRNA processing by RNase E in the pap operon of Escherichia coli.大肠杆菌pap操纵子中核糖核酸酶E对信使核糖核酸加工的体外分析。
Mol Microbiol. 1996 Jul;21(1):55-68. doi: 10.1046/j.1365-2958.1996.6121101.x.
6
Initiation of mRNA decay in Bacillus subtilis.枯草芽孢杆菌中mRNA降解的起始
Mol Microbiol. 1993 Mar;7(5):705-17. doi: 10.1111/j.1365-2958.1993.tb01161.x.
7
Regulation of the packaging of Bacillus thuringiensis delta-endotoxins into inclusions.苏云金芽孢杆菌δ-内毒素包装进内含体的调控
Appl Environ Microbiol. 2001 Nov;67(11):5032-6. doi: 10.1128/AEM.67.11.5032-5036.2001.
8
Decay of a model mRNA in Bacillus subtilis by a combination of RNase J1 5' exonuclease and RNase Y endonuclease activities.枯草芽孢杆菌中 RNase J1 5' 外切酶和 RNase Y 内切酶活性联合导致模型 mRNA 降解。
J Bacteriol. 2011 Nov;193(22):6384-6. doi: 10.1128/JB.05939-11. Epub 2011 Sep 9.
9
SKPDT is a signaling peptide that stimulates sporulation and cry1Aa expression in Bacillus thuringiensis but not in Bacillus subtilis.SKPDT是一种信号肽，可刺激苏云金芽孢杆菌中的孢子形成和cry1Aa表达，但对枯草芽孢杆菌无此作用。
Appl Microbiol Biotechnol. 2007 Aug;76(1):203-9. doi: 10.1007/s00253-007-0982-0. Epub 2007 May 8.
10
Escherichia coli RNase E and RNase G cleave a Bacillus subtilis transcript at the same site in a structure-dependent manner.大肠杆菌核糖核酸酶E和核糖核酸酶G以结构依赖的方式在同一位置切割枯草芽孢杆菌的转录本。
Arch Microbiol. 2004 Feb;181(2):137-43. doi: 10.1007/s00203-003-0637-1. Epub 2003 Dec 19.

引用本文的文献

1
A putative siderophore receptor of 12656-12 under Fur control also binds hemoglobin.在铁摄取调节蛋白（Fur）调控下的一个假定的铁载体受体12656-12也能结合血红蛋白。
Front Microbiol. 2022 Aug 16;13:951173. doi: 10.3389/fmicb.2022.951173. eCollection 2022.
2
The telomerase reverse transcriptase subunit from the dimorphic fungus Ustilago maydis.来自双态真菌玉米黑粉菌的端粒酶逆转录酶亚基。
PLoS One. 2014 Oct 9;9(10):e109981. doi: 10.1371/journal.pone.0109981. eCollection 2014.
3
cry1Aa lacks stability elements at its 5'-UTR but integrity of its transcription terminator is critical to prevent decay of its transcript.cry1Aa在其5'-非翻译区缺乏稳定性元件，但其转录终止子的完整性对于防止其转录本降解至关重要。
Curr Microbiol. 2006 Jul;53(1):23-9. doi: 10.1007/s00284-005-5178-1. Epub 2006 Jun 6.
4
Addition of poly(A) and heteropolymeric 3' ends in Bacillus subtilis wild-type and polynucleotide phosphorylase-deficient strains.枯草芽孢杆菌野生型和多核苷酸磷酸化酶缺陷型菌株中聚腺苷酸和杂聚3'末端的添加
J Bacteriol. 2005 Jul;187(14):4698-706. doi: 10.1128/JB.187.14.4698-4706.2005.
5
RNA processing and degradation in Bacillus subtilis.枯草芽孢杆菌中的RNA加工与降解
Microbiol Mol Biol Rev. 2003 Jun;67(2):157-74, table of contents. doi: 10.1128/MMBR.67.2.157-174.2003.
6
Protection against 3'-to-5' RNA decay in Bacillus subtilis.枯草芽孢杆菌中针对3'至5' RNA降解的保护作用。
J Bacteriol. 1999 Dec;181(23):7323-30. doi: 10.1128/JB.181.23.7323-7330.1999.
7
Decay of ermC mRNA in a polynucleotide phosphorylase mutant of Bacillus subtilis.枯草芽孢杆菌多核苷酸磷酸化酶突变体中ermC信使核糖核酸的衰变
J Bacteriol. 1998 Nov;180(22):5968-77. doi: 10.1128/JB.180.22.5968-5977.1998.

本文引用的文献

1
Processing of the Bacillus subtilis thrS leader mRNA is RNase E-dependent in Escherichia coli.枯草芽孢杆菌苏氨酸合成酶（thrS）前导mRNA的加工在大肠杆菌中依赖于核糖核酸酶E。
J Mol Biol. 1997 May 2;268(2):235-42. doi: 10.1006/jmbi.1997.0971.
2
RNase E: still a wonderfully mysterious enzyme.核糖核酸酶E：仍然是一种极其神秘的酶。
Mol Microbiol. 1997 Mar;23(6):1099-106. doi: 10.1111/j.1365-2958.1997.tb02593.x.
3
Polynucleotide phosphorylase is required for the rapid degradation of the RNase E-processed rpsO mRNA of Escherichia coli devoid of its 3' hairpin.多核苷酸磷酸化酶是大肠杆菌中缺乏3'发夹结构的经核糖核酸酶E加工的rpsO mRNA快速降解所必需的。
Mol Microbiol. 1996 Mar;19(5):997-1005. doi: 10.1046/j.1365-2958.1996.440971.x.
4
Polynucleotide phosphorylase is necessary for competence development in Bacillus subtilis.多核苷酸磷酸化酶对于枯草芽孢杆菌感受态的形成是必需的。
Mol Microbiol. 1996 Jan;19(2):343-56. doi: 10.1046/j.1365-2958.1996.380907.x.
5
STAB-SD: a Shine-Dalgarno sequence in the 5' untranslated region is a determinant of mRNA stability.STAB-SD：5'非翻译区中的Shine-Dalgarno序列是mRNA稳定性的一个决定因素。
Mol Microbiol. 1996 May;20(3):633-43. doi: 10.1046/j.1365-2958.1996.5401046.x.
6
Properties of a Bacillus subtilis polynucleotide phosphorylase deletion strain.枯草芽孢杆菌多核苷酸磷酸化酶缺失菌株的特性
J Bacteriol. 1996 Apr;178(8):2375-82. doi: 10.1128/jb.178.8.2375-2382.1996.
7
Proteins associated with RNase E in a multicomponent ribonucleolytic complex.在多组分核糖核酸酶复合体中与核糖核酸酶E相关的蛋白质。
Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):3865-9. doi: 10.1073/pnas.93.9.3865.
8
A DEAD-box RNA helicase in the Escherichia coli RNA degradosome.大肠杆菌RNA降解体中的一种DEAD盒RNA解旋酶。
Nature. 1996 May 9;381(6578):169-72. doi: 10.1038/381169a0.
9
A protein complex mediating mRNA degradation in Escherichia coli.一种介导大肠杆菌中mRNA降解的蛋白质复合物。
Mol Microbiol. 1994 Nov;14(4):717-29. doi: 10.1111/j.1365-2958.1994.tb01309.x.
10
A human RNase E-like activity that cleaves RNA sequences involved in mRNA stability control.一种切割参与mRNA稳定性控制的RNA序列的人源核糖核酸酶E样活性。
Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7322-6. doi: 10.1073/pnas.92.16.7322.

枯草芽孢杆菌中cry1Aa mRNA的衰变机制。

Mechanism of decay of the cry1Aa mRNA in Bacillus subtilis.

作者信息

Vázquez-Cruz C, Olmedo-Alvarez G

机构信息

Departamento de Ingeniería Genética, CINVESTAV IPN, Unidad Irapuato, Guanajuato, México.

出版信息

J Bacteriol. 1997 Oct;179(20):6341-8. doi: 10.1128/jb.179.20.6341-6348.1997.

DOI:10.1128/jb.179.20.6341-6348.1997
PMID:9335281
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC179548/
Abstract

We undertook the study of the decay process of the cry1Aa mRNA of Bacillus thuringiensis expressed in B. subtilis. The cry1Aa transcript is a 3.7-kb mRNA expressed during sporulation whose transcriptional control has previously been studied in both B. subtilis and B. thuringiensis. We found that the cry1Aa mRNA has a half-life of around 9 min and that its decay occurs through endoribonucleolytic cleavages which result in three groups of high-molecular-weight mRNA intermediates ranging in size from 2.7 to 0.5 kb. A comparative study carried out with Escherichia coli showed a similar pattern of degradation intermediates. Primer extension analysis carried out on RNA from B. subtilis revealed that most cleavages occur within two regions located toward the 5' and 3' ends of the mRNA. The most prominent processing site observed for the cry1Aa mRNA isolated from B. subtilis is only two bases away from that occurring on RNA isolated from E. coli. Most cleavage sites occur at seemingly single-stranded RNA segments rich in A and U nucleotides, suggesting that a common and conserved mechanism may process the cry1Aa mRNA.

摘要

我们对在枯草芽孢杆菌中表达的苏云金芽孢杆菌cry1Aa mRNA的衰变过程进行了研究。cry1Aa转录本是一种在芽孢形成过程中表达的3.7 kb mRNA，其转录调控此前已在枯草芽孢杆菌和苏云金芽孢杆菌中进行过研究。我们发现cry1Aa mRNA的半衰期约为9分钟，其衰变通过核糖核酸内切酶切割发生，这导致产生三组高分子量mRNA中间体，大小从2.7 kb到0.5 kb不等。与大肠杆菌进行的比较研究显示出类似的降解中间体模式。对来自枯草芽孢杆菌的RNA进行的引物延伸分析表明，大多数切割发生在mRNA 5'端和3'端附近的两个区域内。从枯草芽孢杆菌分离的cry1Aa mRNA观察到的最突出加工位点与从大肠杆菌分离的RNA上的位点仅相差两个碱基。大多数切割位点出现在富含A和U核苷酸的看似单链RNA片段上，这表明可能存在一种共同且保守的机制来加工cry1Aa mRNA。