Chantes-Guerra Alberto, Maldonado-Puga Samantha, Rojas-Ruiz Norma, Rea-Hernandez Ismael, Montes-Garcia Fernando J, Trujillo-Ruiz Hector, Yañez-Aguilar Ivan E, Vazquez-Cruz Candelario, Sanchez-Alonso Patricia, Negrete-Abascal Erasmo
Instituto de Ciencias, Benemérita Universidad Autonóma de Puebla, Puebla, Mexico.
Centro de Investigaciones y Estudios Avanzados, Instituto Politécnico Nacional de México (CINVESTAV), Mexico City, Mexico.
Front Microbiol. 2022 Aug 16;13:951173. doi: 10.3389/fmicb.2022.951173. eCollection 2022.
family members obtain iron directly from host proteins or through siderophore-dependent mechanisms. Although expresses different virulence factors, its response to growth under iron restriction is unknown. cultured in the presence of 2,2'-dipyridyl, up-expressed an approximately 65 kDa protein and repressed the expression of a 70 kDa protein. MALDI-TOF analysis of those proteins indicated homology with CirA (65 kDa), a protein involved in iron-siderophore acquisition in and a TonB-dependent receptor (70 kDa protein), a protein that binds chicken hemoglobin; however, siderophore production was not detected by chromo azurol S (CAS)-BHI agar determination. This putative siderophore receptor is under Fur control, but not the hemoglobin binding protein, as observed in 12656-12 mutant (Ω 126.13) grown in the presence or not of 2,2'-dipyridyl. The addition of FeCl to the culture medium diminished the growth and biofilm production in approximately 30% and 35%, respectively, in the wild-type strain, but the growth of Ω 126.13 strain was not affected and biofilm production increased in 35%. Ω 126.13 presented lower virulence when it was inoculated to 35-day-old chickens in comparison to the wild-type strain. The induction of more than one iron uptake mechanism could benefit pathogenic microorganisms such as .
家庭成员直接从宿主蛋白中获取铁，或通过依赖铁载体的机制获取铁。尽管[具体微生物名称未给出]表达不同的毒力因子，但其在铁限制条件下的生长反应尚不清楚。在2,2'-联吡啶存在的情况下培养[具体微生物名称未给出]，其上调表达了一种约65 kDa的蛋白质，并抑制了一种70 kDa蛋白质的表达。对这些蛋白质的基质辅助激光解吸电离飞行时间（MALDI-TOF）分析表明，它们与CirA（65 kDa）具有同源性，CirA是一种参与[具体微生物名称未给出]中铁-铁载体获取的蛋白质，还与一种TonB依赖性受体（70 kDa蛋白质）具有同源性，该受体可结合鸡血红蛋白；然而，通过铬天青S（CAS）-脑心浸液（BHI）琼脂测定未检测到[具体微生物名称未给出]产生铁载体。如在2,2'-联吡啶存在或不存在的情况下培养的12656-12突变体（Ω126.13）中所观察到的，这种假定的[具体微生物名称未给出]铁载体受体受铁摄取调节因子（Fur）控制，但血红蛋白结合蛋白不受其控制。向培养基中添加氯化铁（FeCl）分别使野生型菌株的生长和生物膜产生减少了约30%和35%，但Ω126.13菌株的生长未受影响，且生物膜产生增加了35%。与野生型菌株相比，将Ω126.13接种到35日龄鸡中时，其毒力较低。诱导多种铁摄取机制可能有益于诸如[具体微生物名称未给出]这样的致病微生物。
